Comprehensive Sequence and Whole-Life-Cycle Expression Profile Analysis of the Phosphate Transporter Gene Family in Rice
Comprehensive Sequence and Whole-Life-Cycle Expression Profile Analysis of the Phosphate Transporter Gene Family in Rice
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DOI：
10.1093/mp/ssr058
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发表时间：
2011-11-01
期刊：
影响因子：
27.5
通讯作者：
Lian, Xing-Ming
中科院分区：
文献类型：
--
作者：
Liu, Fang;Chang, Xiao-Jian;Lian, Xing-Ming
植物磷酸转运蛋白(PT)基因是一个在多种生理生化过程中发挥重要作用的大家族。在本研究中，通过数据库搜索，获得了26个水稻潜在的PT家族基因。对这些基因的分析得到了8个保守基序和5-12个跨膜片段，其中大部分是保守的。在这些基因的2kb上游区域，共发现237个可能的顺式元件。其中大部分是PI反应元件和其他与胁迫相关的顺式调控元件，如pho-like、Tata-box-like、Phr1或Helix-loop-Helix元件，以及WRKY1和Abre元件，表明这些信号对基因的调控。利用从明恢63、珍杉97和汕优63三个水稻品种的27个组织的RNA杂交的芯片数据，对这些基因的全面表达进行了分析。实时荧光定量PCR分析证实，3个PT基因在开花前1d在雄蕊中优先表达，2个在抽穗期在穗部表达，2个在抽穗后14d在剑叶中优先表达。激素处理实验表明，在5种激素作用下，水稻幼苗中的11个PT基因分别有不同程度的上调或下调。这些结果将有助于阐明这些基因在水稻生长、发育和逆境反应中的作用。
Plant phosphate transporter (PT) genes comprise a large family with important roles in various physiological and biochemical processes. In this study, a database search yielded 26 potential PT family genes in rice (Oryza sativa). Analysis of these genes led to identification of eight conserved motifs and 5-12 trans-membrane segments, most of them conserved. A total of 237 putative cis elements were found in the 2-kb upstream region of these genes. Of these, a majority were Pi-response and other stress-related cis regulatory elements, such as PHO-like, TATA-box-like, PHR1, or Helix-loop-helix elements, and WRKY1 and ABRE elements, suggesting gene regulation by these signals. Comprehensive expression analysis of these genes was performed using data from microarrays hybridized with RNA from 27 tissues covering the entire lifecycle from three rice genotypes: Minghui 63, Zhenshan 97, and Shanyou 63. Real-time PCR analysis confirmed that three rice PT genes are preferentially expressed in stamen at 1 d before flowering, two in panicle at the heading stage, and two in flag leaf at 14 d after the heading stage. Hormone-treatment experiments revealed differential up-regulation or down-regulation of 11 rice PT genes in seedlings exposed to five hormones, respectively. These results will be useful for elucidating the roles of these genes in the growth, development, and stress response of the rice plant.