Aquaporins in ovine amnion: responses to altered amniotic fluid volumes and intramembranous absorption rates.
Aquaporins in ovine amnion: responses to altered amniotic fluid volumes and intramembranous absorption rates.
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DOI：
10.14814/phy2.12868
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发表时间：
2016-07
影响因子：
2.5
通讯作者：
Brace RA
中科院分区：
文献类型：
--
作者：
Cheung CY;Anderson DF;Brace RA
水通道蛋白（AQPs）是一种跨膜通道蛋白，可以促进水在细胞膜上的快速运动。在羊膜中，AQP‐促进水在羊膜细胞间的转移被认为是羊水体积（AFV）调节的一种机制。为了研究AQP是否通过改变膜内吸收（IMA）率来调节AFV，我们在实验条件下，在IMA率和AFV的大范围修饰下，验证了羊膜中AQP基因表达与IMA率正相关的假设。在对照组、排尿、排尿或羊水输注2 d的16只妊娠晚期羊胎羊膜中，测定了AQP1、AQP3、AQP8、AQP9和AQP11 mRNA和蛋白的相对丰度。RT - qPCR检测AQP mRNA水平，western免疫印迹检测AQP蛋白水平。在对照条件下，5种AQPs的mRNA水平差异超过20倍。在实验治疗期间，实验组的平均IMA率为100±120 mL/d ~ 1370±270 mL/d。与对照组相比，5种AQPs的mRNA水平没有变化，也与IMA发生率无关。AQP1蛋白水平与IMA发生率呈正相关（r 2 = 38%, P = 0.01），其余4个aqp蛋白水平与IMA发生率无显著正相关。上述结果表明，绵羊羊膜中存在5种AQPs的差异表达。我们的研究支持了AQP1可能在调节羊膜内液体传输速率中发挥积极作用，从而参与AFV的动态调节的假设。
Aquaporins (AQPs) are transmembrane channel proteins that facilitate rapid water movement across cell membranes. In amniotic membrane, the AQP‐facilitated transfer of water across amnion cells has been proposed as a mechanism for amniotic fluid volume (AFV) regulation. To investigate whether AQPs modulate AFV by altering intramembranous absorption (IMA) rate, we tested the hypothesis that AQP gene expression in the amnion is positively correlated with IMA rate during experimental conditions when IMA rate and AFV are modified over a wide range. The relative abundances of AQP1, AQP3, AQP8, AQP9, and AQP11 mRNA and protein were determined in the amnion of 16 late‐gestation ovine fetuses subjected to 2 days of control conditions, urine drainage, urine replacement, or intraamniotic fluid infusion. AQP mRNA levels were determined by RT‐qPCR and proteins by western immunoblot. Under control conditions, mRNA levels among the five AQPs differed more than 20‐fold. During experimental treatments, mean IMA rate in the experimental groups ranged from 100 ± 120 mL/day to 1370 ± 270 mL/day. The mRNA levels of the five AQPs did not change from control and were not correlated with IMA rates. The protein levels of AQP1 were positively correlated with IMA rates (r 2 = 38%, P = 0.01) while the remaining four AQPs were not. These findings demonstrate that five AQPs are differentially expressed in ovine amnion. Our study supports the hypothesis that AQP1 may play a positive role in regulating the rate of fluid transfer across the amnion, thereby participating in the dynamic regulation of AFV.