Performance comparison of microbial fuel cells equipped with different membrane electrode assemblies
Performance comparison of microbial fuel cells equipped with different membrane electrode assemblies
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DOI：
10.1088/1742-6596/433/1/012022
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发表时间：
2013-04
期刊：
影响因子：
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通讯作者：
O. Rubaba;Y. Araki;S. Yamamoto;K. Suzuki;H. Sakamoto;A. Matsuda;H. Futamata
中科院分区：
文献类型：
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作者：
O. Rubaba;Y. Araki;S. Yamamoto;K. Suzuki;H. Sakamoto;A. Matsuda;H. Futamata
微生物燃料电池（MFC）的实际应用不仅需要开发新的材料，包括电极和质子交换膜，还需要了解与发电相关的细菌群落结构。在这里，四种新型的膜电极组件（MEA）。四个乳酸美联储配备的膜的MFC，其特征在于通过电化学，分子依赖性和分子无关的方法。配备Nafion117型MEA（18 μm厚度）的MFC 1表现出最高的性能。聚二烯丙基二甲基氯化铵、聚烯丙基胺盐酸盐和聚丙烯酰胺基2甲基丙磺酸三种不同构型的膜电极厚度分别为0.3 μm（MEA 2和MEA 3）和1.0 μm（MEA 4），但其功率密度较低。变性梯度凝胶电泳（DGGE）和系统发育分析表明，厌氧细菌占主导地位的阳极生物膜MFC 1。从MFC 1的阳极生物膜中分离出与DGGE条带之一的核苷酸序列完全对应的细菌。有趣的是，BLAST搜索表明该细菌（命名为菌株RO 1）属于革兰氏阳性菌属，丙酸杆菌属。在纯培养的MFC中，菌株RO 1能够产电并在阳极表面构建生物膜。这些结果表明，MEA的性质显着影响细菌群落结构，从而影响MFC的性能。
It is important for practical use of microbial fuel cells (MFCs) to not only develop new materials including electrodes and proton exchange membranes but also to understand the bacterial community structure related to electricity generation. Here, four kinds of novel membrane electrode assemblies (MEAs) were made. Four lactate fed MFCs equipped with the membranes were characterized by electrochemical, molecular-dependent and molecular-independent methods. MFC1 equipped with Nafion 117-type MEA (18 μm thickness) exhibited the highest performance. Although the other MEAs with different configurations of three kinds of polymers; poly (diallyldimethylammonium chloride), polyallylamine hydrochloride and poly (2-acrylamino-2-methyl -1-propanesulfonic acid) had thicknesses of about 0.3 μm (MEA 2 and 3) and 1.0 μm (MEA4), their power densities were lower. Denaturing gradient gel electrophoresis (DGGE) and phylogenetic analyses showed that anaerobic bacteria dominated in anode biofilms of MFC1. A bacterium completely corresponding to nucleotide sequence of one of the DGGE bands was isolated from the anode biofilm in MFC1. Interestingly, BLAST search indicated that the bacterium (named strain RO1) belonged to the genus of gram positive bacterium, Propioniferax. It was confirmed that strain RO1 was capable of producing electricity and constructing biofilm on the anode surface in pure culture MFC. These results suggested that the property of MEA affects significantly the bacterial community structure, thereby influencing the MFC-performance.