Inhibition of measles virus infection and fusion with peptides corresponding to the leucine zipper region of the fusion protein
Inhibition of measles virus infection and fusion with peptides corresponding to the leucine zipper region of the fusion protein
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DOI：
10.1099/0022-1317-78-1-107
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发表时间：
1997-01-01
影响因子：
3.8
通讯作者：
Buckland, R
中科院分区：
文献类型：
--
作者：
Wild, TF;Buckland, R
麻疹病毒（MV）感染的特征在于合胞体的诱导，即感染细胞的融合。两种MV蛋白，血凝素（HA）和融合（F）蛋白，参与了这一过程。研究了代表MV F蛋白的两个α-螺旋区域的合成肽抑制MV融合的能力。对应于亮氨酸拉链区（氨基酸455-490）的肽抑制MV融合，而对应于两亲性α-螺旋区的氨基酸148-177的肽不抑制MV融合。融合抑制也得到了与牛痘病毒表达的HA和F，最近的野生型MV分离株和密切相关的犬瘟热病毒，但不是与腮腺炎病毒。F455-490肽不影响MV F的合成或其向细胞膜的转运。病毒细胞附着不受影响，但溶血和病毒进入细胞受到抑制。在一步生长曲线中，病毒产量不受影响。
Measles virus (MV) infections are characterized by the induction of syncytia, i.e. the fusion of infected cells. Two MV proteins, the haemagglutinin (HA) and fusion (F) proteins, are involved in this process. Synthetic peptides representing two alpha-helical regions of the MV F protein were studied for their ability to inhibit MV fusion. A peptide corresponding to the leucine zipper region (amino acids 455-490) inhibited MV fusion, whereas a peptide to amino acids 148-177, corresponding to the amphipathic alpha-helix region, did not. Fusion inhibition was also obtained with vaccinia virus-expressed HA and F, a recent wild-type MV isolate and the closely related canine distemper virus, but not with mumps virus. The F455-490 peptide did not affect the synthesis of MV F or its transport to the cell membrane. Virus-cell attachment was unaffected, but haemolysis and virus entry into the cell were inhibited. In one-step growth curves the virus yield was unaffected.