The CLAVATA and SHOOT MERISTEMLESS loci competitively regulate meristem activity in Arabidopsis.
The CLAVATA and SHOOT MERISTEMLESS loci competitively regulate meristem activity in Arabidopsis.
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发表时间：
1996-05
期刊：
影响因子：
4.6
通讯作者：
S. Clark;S. Jacobsen;J. Levin;E. Meyerowitz
中科院分区：
文献类型：
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作者：
S. Clark;S. Jacobsen;J. Levin;E. Meyerowitz
CLAVATA（CLV1 和 CLV3）和 SHOOT MERISTEMLESS (STM) 基因专门调节拟南芥中芽分生组织的发育。 CLV和STH似乎具有相反的功能：c1v1和Clv3突变体在芽和花分生组织中积累过量的未分化细胞，而stm突变体在胚胎发育过程中无法形成芽分生组织的未分化细胞。我们发现了 stm 的一个弱等位基因（stm-2），它表明 STM 不仅是枝条分生组织建立所必需的，而且也是枝条分生组织中未分化细胞持续维持和花分生组织中细胞正常增殖所必需的。我们发现了 CLV 和 STM 位点之间遗传相互作用的证据。 clv1 和 c1v3 突变部分抑制 stm-1 和 stm-2 表型，并且能够以显性方式抑制。 clv stm双突变体和stm纯合但clv杂合的植物虽然仍然缺乏胚芽分生组织，但表现出大大增强的胚后芽和花分生组织发育。尽管stm表型是隐性的，但stm突变显性抑制clv纯合和杂合表型。这些结果表明stm表型对CLV活性水平敏感，而clv表型对STM活性水平敏感。我们认为这些基因在芽和花分生组织的细胞分裂和/或细胞分化的调节中发挥相关但相反的作用。
The CLAVATA (CLV1 and CLV3) and SHOOT MERISTEMLESS (STM) genes specifically regulate shoot meristem development in Arabidopsis. CLV and STH appear to have opposite functions: c1v1 and Clv3 mutants accumulate excess undifferentiated cells in the shoot and floral meristem, while stm mutants fail to form the undifferentiated cells of the shoot meristem during embryonic development. We have identified a weak allele of stm (stm-2) that reveals STM is not only required for the establish- ment of the shoot meristem, but is also required for the continued maintenance of undifferentiated cells in the shoot meristem and for proper proliferation of cells in the floral meristem. We have found evidence of genetic interactions between the CLV and STM loci. clv1 and c1v3 mutations partially suppressed the stm-1 and stm-2 phenotypes, and were capable of suppression in a dominant fashion. clv stm double mutants and plants homozygous for stm but heterozygous for clv, while still lacking an embryonic shoot meristem, exhibited greatly enhanced postembryonic shoot and floral meristem development. Although stm phenotypes are recessive, stm mutations dominantly suppressed clv homozygous and heterozygous phenotypes. These results indicate that the stm phenotype is sensitive to the levels of CLV activity, while the clv phenotype is sensitive to the level of STM activity. We propose that these genes play related but opposing roles in the regulation of cell division and/or cell differentiation in shoot and floral meristems.