The effects of heavy resistance training and detraining on satellite cells in human skeletal muscles
The effects of heavy resistance training and detraining on satellite cells in human skeletal muscles
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DOI：
10.1113/jphysiol.2004.065904
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发表时间：
2004-08-01
影响因子：
5.5
通讯作者：
Andersen, JL
中科院分区：
文献类型：
--
作者：
Kadi, F;Schjerling, P;Andersen, JL
本研究的目的是探讨在30和90天的抗阻训练和3，10，30，60和90天的停训后，卫星细胞含量和肌细胞数量的调制。从15名年轻男性（平均年龄：24岁;范围：20-32岁）的股外侧肌获得肌肉活检。卫星细胞和肌细胞核进行了研究，用抗CD 56的单克隆抗体染色的肌肉横截面和迈耶的苏木精复染。北方印迹法检测细胞周期标志物CyclinD 1和p21 mRNA水平。卫星细胞含量在30天增加了19%（P = 0.02），在90天增加了31%（P = 0.0003）。与训练前的值相比，卫星细胞的数量在3、10和60天仍然显著升高，但在停训90天时没有。两种细胞周期标志物CyclinD 1和p21 mRNA在30天的训练中显著增加。在90天的训练中，p21仍然升高，而CyclinD 1恢复到训练前的值。在停训期，p21和CyclinD 1水平与训练前值相似。有没有显着的变化，在训练和停训期后的肌细胞核的数量。在整个训练期间，每个肌核控制的纤维面积逐渐增加，并在停训期间恢复到训练前的值。总之，这些结果表明，卫星细胞在响应训练和停训刺激的高可塑性，并清楚地表明，骨骼肌纤维的大小可以实现适度的变化，而无需添加新的肌细胞核。
The aim of this study was to investigate the modulation of satellite cell content and myonuclear number following 30 and 90 days of resistance training and 3,10,30,60 and 90 days of detraining. Muscle biopsies were obtained from the vastus lateralis of 15 young men (mean age: 24 years; range: 20-32 years). Satellite cells and myonuclei were studied on muscle cross-sections stained with a monoclonal antibody against CD56 and counterstained with Mayer's haematoxylin. Cell cycle markers CyclinD1 and p21 mRNA levels were determined by Northern blotting. Satellite cell content increased by 19% (P = 0.02) at 30 days and by 31% (P = 0.0003) at 90 days of training. Compared to pre-training values, the number of satellite cells remained significantly elevated at 3, 10 and 60 days but not at 90 days of detraining. The two cell cycle markers CyclinD1 and p21 mRNA significantly increased at 30 days of training. At 90 days of training, p21 was still elevated whereas CyclinD1 returned to pre-training values. In the detraining period, p21 and CyclinD1 levels were similar to the pre-training values. There were no significant alterations in the number of myonuclei following the training and the detraining periods. The fibre area controlled by each myonucleus gradually increased throughout the training period and returned to pre-training values during detraining. In conclusion, these results demonstrate the high plasticity of satellite cells in response to training and detraining stimuli and clearly show that moderate changes in the size of skeletal muscle fibres can be achieved without the addition of new myonuclei.