Resonance assignment of methionine methyl groups and χ3angular information from long-range proton—carbon and carbon—carbon J correlation in a calmodulin—peptide complex
Resonance assignment of methionine methyl groups and χ3angular information from long-range proton—carbon and carbon—carbon J correlation in a calmodulin—peptide complex
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钙调蛋白-肽复合物中蛋氨酸甲基的共振分配和长程质子-碳和碳-碳 J 相关性的 χ3 角度信息
DOI：
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发表时间：
1994
影响因子：
2.7
通讯作者：
G. W. Vuister
中科院分区：
文献类型：
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作者：
A. Bax;F. Delaglio;S. Grzesiek;G. W. Vuister
通过几个简单的3D实验，给出了甲硫氨酸C ε甲基碳与C γ H2质子或C β和C γ之间的J相关性。J关联的强度提供了关于三键J耦合的大小的信息，从而提供了关于χ 3扭转角的信息。此外，HMBC实验的简单3D版本提供了C ε H3甲基质子和C γ之间的灵敏联系。该方法被证明为20 kDa的钙调蛋白和骨骼肌肌球蛋白轻链激酶的26个残基的肽片段之间的复合物。
SummarySeveral simple 3D experiments are used to provide J correlations between methionine Cε methyl carbons and either the CγH2 protons or Cβ and Cγ. The intensity of the J correlations provides information on the size of the three-bond J couplings and thereby on the χ3 torsion angle. In addition, a simple 3D version of the HMBC experiment provides a sensitive link between the CεH3 methyl protons and Cγ. The methods are demonstrated for a 20 kDa complex between calmodulin and a 26-residue peptide fragment of skeletal muscle myosin light chain kinase.