Formation of fluorescent products in the reaction of adenine and other bases with secondary oxidation products of methyl linoleate hydroperoxides.
Formation of fluorescent products in the reaction of adenine and other bases with secondary oxidation products of methyl linoleate hydroperoxides.
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腺嘌呤和其他碱与亚油酸甲酯氢过氧化物的二次氧化产物反应形成荧光产物。
DOI：
10.1271/bbb1961.53.1575
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
E. Frankel
中科院分区：
文献类型：
--
作者：
K. Hasegawa;K. Fujimoto;T. Kaneda;W. Neff;E. Frankel
通过测定抗坏血酸和Fe2 +存在下亚油酸甲酯氢过氧化物与DNA、核苷和DNA碱基的荧光形成，研究了亚油酸甲酯氢过氧化物与DNA的反应。亚油酸甲酯氢过氧化物仅对腺苷和DNA产生显著的荧光，而对其他核苷没有荧光。腺嘌呤也产生了类似的荧光，表明6位的氨基优先与脂质氧化产物反应产生荧光。在4.0 ~9.0范围内，pH水平对荧光强度和荧光光谱均无明显影响。在亚油酸甲酯氢过氧化物的降解产物中，2-辛烯醛和2,4-十二烯醛即使在没有FeS04和抗坏血酸的情况下也能与腺嘌呤产生荧光。然而，这些羰基化合物在与DNA、FeS04和抗坏血酸反应时都没有产生明显的荧光。碘化钾、对苯二酚、α -生育酚和BHA降低了氟的含量。
The reaction of methyl linoleate hydroperoxides with DNA, nucleosides and DNA bases was investigated by determining the fluorescence formation in the presence of ascorbic acid and Fe2 +. Methyl linoleate hydroperoxides produced significant fluorescence only with adenosine and with DNA, but not with the other nucleosides tested. Adenine also produced similar fluorescence, suggesting that the amino group at the 6-position reacted preferentially with the lipid oxidation products to produce fluorescence. The pH level did not have any distinct effect on either the intensity or spectrum of fluorescence in the range of 4.0 ~9.0. Among the degradation products of methyl linoleate hydroperoxides, 2-octenal and 2,4-decadienal produced fluorescence with adenine even in the absence of FeS04 and ascorbic acid. However, none of these carbonyl compounds produced significant fluorescence in the reaction with DNA, FeS04 and ascorbic acid. Potassium iodide, hydroquinone, alpha-tocopherol and BHA reduced the amount of fluor...