Porcine regulatory T cells: Mechanisms and T-cell targets of suppression
Porcine regulatory T cells: Mechanisms and T-cell targets of suppression
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DOI：
10.1016/j.dci.2011.04.006
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发表时间：
2011-11-01
影响因子：
2.9
通讯作者：
Saalmueller, Armin
中科院分区：
文献类型：
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作者：
Kaeser, Tobias;Gerner, Wilhelm;Saalmueller, Armin
已知睾酮对各种免疫反应具有抑制能力。在猪中，可以证明Foxp 3（+）T β的存在以及抑制活性，但缺乏详细的功能研究。因此，我们分析了猪胸腺肽的功能特性。我们观察到除了TCR刺激之外，TCR还需要IL-2来激活。此外，我们研究了以下抑制机制：（i）细胞-细胞接触依赖性，（ii）可溶性抑制因子的产生和（iii）生长因子的竞争。我们的实验表明，通过阻断细胞-细胞接触或通过补充过量的IL-2，猪T细胞的抑制被废除。另外，可以表明猪THBE产生免疫抑制性IL-10。因此，我们证明了猪THBE可以使用上述所有主要的抑制机制。使用CFSE增殖测定法对TcR的抑制活性的进一步研究表明，抑制作用影响T辅助细胞以及细胞毒性T淋巴细胞和TCR-γ δ T细胞。(C)2011爱思唯尔有限公司保留所有权利。
Tregs are known for their suppressive capacity on various immune reactions. In swine, existence as well as suppressive activity of Foxp3(+) Tregs could be demonstrated but detailed functional investigations are lacking. Therefore, we analysed the functional properties of porcine Tregs. We observed that besides TCR stimulation Tregs require IL-2 for activation. Furthermore, we investigated the following mechanisms of suppression: (i) cell-cell contact dependency, (ii) production of soluble suppressive factors and (iii) competition for growth factors. Our experiments revealed that suppression by porcine Tregs is abrogated by blocking cell-cell contact or by supplementing excessive amounts of IL-2. Additionally it could be shown that porcine Tregs produce immunosuppressive IL-10. Thereby, we demonstrated that porcine Tregs can use all main mechanisms of suppression mentioned above. Further investigations on the suppressive activity of Tregs using CFSE proliferation assays demonstrated that suppression affects T-helper cells as well as cytotoxic T lymphocytes and TCR-gamma delta T cells. (C) 2011 Elsevier Ltd. All rights reserved.