Adsorption and Release Behaviors of Vascular Endothelial Growth Factor on Porous Hydroxyapatite Ceramic Under Competitive Conditions
Adsorption and Release Behaviors of Vascular Endothelial Growth Factor on Porous Hydroxyapatite Ceramic Under Competitive Conditions
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DOI：
10.1166/jbt.2014.1144
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发表时间：
2014-02-01
影响因子：
0.1
通讯作者：
Zhang, Xingdong
中科院分区：
文献类型：
--
作者：
Chen, Ying;Wang, Jing;Zhang, Xingdong
血管内皮生长因子在骨移植后的血管化过程中起着积极的作用。本研究构建了一个多蛋白质体系，并在竞争条件下研究了其在多孔羟基磷灰石陶瓷上的吸附和释放，并通过细胞增殖实验评价了其生物活性。结果表明，血管内皮生长因子在多孔羟基磷灰石陶瓷上始终具有较高的吸附量，无论是否有其他血清蛋白的抑制作用。当在含有10%胎牛血清的细胞培养基中孵育时，预载的血管内皮生长因子显示出从陶瓷缓慢而持续的释放。培养7天后，其累积释放率仅为50%左右。血管内皮生长因子在多孔羟基磷灰石陶瓷上的高吸附和缓慢释放应归因于它们之间的强静电引力以及陶瓷表面存在的丰富的纳米尺寸的微孔。细胞培养实验证实，多孔羟基磷灰石陶瓷释放的血管内皮生长因子能显著促进人脐静脉内皮细胞的增殖，表明其脱附后仍保持生物活性。因此，可以推断多孔羟基磷灰石陶瓷可能在植入早期吸附并积累了内源性血管内皮生长因子，然后从陶瓷表面逐渐释放并作用于向内生长的血管内皮细胞，导致植入物快速血管化。
Vascular endothelial growth factor plays a positive role in the vascularization of bone grafts after implantation. In the present study, we constructed a multi-protein system and investigated its adsorption and release on porous hydroxyapatite ceramic under competitive conditions, and we also evaluated its bioactivity by cells proliferation experiments after it released from the ceramic. The results showed that vascular endothelial growth factor always had a high adsorption on porous hydroxyapatite ceramic, whether having the inhibition of other serum proteins or not. When incubated in the cell culture medium containing 10% fetal calf serum, the pre-loaded vascular endothelial growth factor showed a slow and sustained release from the ceramic. After 7 days of incubation, its accumulative release rate was only about 50%. The high adsorption and slow release of vascular endothelial growth factor on porous hydroxyapatite ceramic should be ascribed to the strong electrostatic attraction between them as well as the abundant nano-sized micropores present on the ceramic surface. The cell culture experiments confirmed that the released vascular endothelial growth factor from porous hydroxyapatite ceramic could promote the proliferation of human umbilical vein endothelial cells significantly, indicating that its bioactivity was still maintained after desorbing from the ceramic. As thus, it could be inferred that porous hydroxyapatite ceramic may adsorb and accumulate the endogenous vascular endothelial growth factor at the early implantation stage, which then releases from the ceramic surface gradually and takes effect on the ingrown vascular endothelial cells, leading to the rapid vascularization of the implant.