Diversity of virus-like agents killing Microcystis aeruginosa in a hyper-eutrophic pond
Diversity of virus-like agents killing Microcystis aeruginosa in a hyper-eutrophic pond
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DOI：
10.1093/plankt/fbi128
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发表时间：
2006-04-01
影响因子：
2.1
通讯作者：
Kawabata, Z
中科院分区：
文献类型：
--
作者：
Honjo, M;Matsui, K;Kawabata, Z
我们证明了存在的多样性的病毒样药物杀死铜绿微囊藻在日本的浅富营养化池塘，但没有特定的病毒分离，一次在2001年10月和两次在12月。用氯仿处理池水，通过GF-75过滤器（0.3 μ m）过滤，然后与无菌M.铜绿假单胞菌7天。M.铜绿假单胞菌在孵育过程中减少了10倍。结果表明，细胞死亡的活性剂是病毒样的，根据其大小部分（从30 kDa到0.3 μ m），对热处理的敏感性，以及在我们的提取过程中DNA的蛋白质保护的证据。从我们的脉冲场凝胶电泳（PFGE）分析的结果，两个或四个不同大小的DNA条带出现在每个实验中的培养裂解液。从三个样品中总共观察到10条大小不同的条带（10-90 kb），表明多种病毒样因子杀死M.铜绿假单胞菌在该池塘中存在。据我们所知，这是第一次报道存在不同基因组大小的病毒样因子杀死M。铜绿。
We demonstrated the existence of the diversity of virus-like agents killing Microcystis aeruginosa in a shallow hyper-eutrophic pond in Japan, but without specific virus isolation, once in October and twice in December 2001. The pond water was treated by chloroform, filtered throughout GF-75 filter (0.3 mu m) and then incubated with axenic M. aeruginosa for 7 days. The cell density of M. aeruginosa decreased 10-fold in the course of the incubation. Results suggested that the active agent for cell death was virus-like, based upon its size fraction (from 30 kDa to 0.3 mu m), sensitivity on heat-treatment, and evidence of protein protection of DNA during our extraction procedure. From our results of pulsed field gel electrophoresis (PFGE) analysis, two or four different-sized DNA bands appeared from the culture lysate in each experiment. In total, 10 different-sized bands (10-90 kb) were observed from the three sample dates, suggesting that multiple virus-like agents killing M. aeruginosa existed in this pond. To our knowledge, this is the first report of the existence of virus-like agents with different genomic size that kill M. aeruginosa.