Tissue-specific regulation of the LIM homeobox gene lin-11 during development of the Caenorhabditis elegans egg-laying system
Tissue-specific regulation of the LIM homeobox gene lin-11 during development of the Caenorhabditis elegans egg-laying system
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DOI：
10.1006/dbio.2002.0688
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发表时间：
2002-07-01
影响因子：
2.7
通讯作者：
Sternberg, PW
中科院分区：
文献类型：
--
作者：
Gupta, BP;Sternberg, PW
秀丽隐杆线虫雌雄同体的产卵系统需要外阴的发育及其与子宫的精确连接。该过程受let -23介导的表皮生长因子信号通路和lin -12介导的侧通路调控。在这些途径下游的核因子中，LIM同源盒基因lin-11起着主要作用。由于外阴系和子宫缝细胞形成的异常，Lin-11突变动物存在产蛋缺陷。然而，提供lin-11功能特异性的机制尚不清楚。在这里，我们研究了在产卵系统发育过程中lin-11的调控。我们的结果表明，lin-11的组织特异性表达是由两个不同的调控元件控制的，它们作为独立的模块起作用，共同指定了一个野生型产卵系统。子宫pi谱系模块依赖于LIN-12/Notch信号，而外阴模块依赖于lin -17介导的Wnt信号。这些结果提供了一个独特的例子，通过两个进化上保守的信号通路组织特异性调节一个LIM同源盒基因。最后，我们提供的证据表明，LIN-12/Notch信号对lin-11的调控是由Su(H)/CBF1家族成员LAG-1直接介导的。(C) 2002 Elsevier Science （USA）。
The egg-laying system of Caenorhabditis elegans hermaphrodites requires development of the vulva and its precise connection with the uterus. This process is regulated by LET-23-mediated epidermal growth factor signaling and LIN-12-mediated lateral signaling pathways. Among the nuclear factors that act downstream of these pathways, the LIM homeobox gene lin-11 plays a major role. lin-11 mutant animals are egg-laying defective because of the abnormalities in vulval lineage and uterine seam- cell formation. However, the mechanisms providing specificity to lin-11 function are not understood. Here, we examine the regulation of lin-11 during development of the egg-laying system. Out results demonstrate that the tissue-specific expression of lin-11 is controlled by two distinct regulatory elements that function as independent modules and together specify a wild-type egg-laying system. A uterine pi lineage module depends on the LIN-12/Notch signaling, while a vulval module depends on the LIN-17-mediated Wnt signaling. These results provide a unique example of the tissue-specific regulation of a LIM homeobox gene by two evolutionarily conserved signaling pathways. Finally, we provide evidence that the regulation of lin-11 by LIN-12/Notch signaling is directly mediated by the Su(H)/CBF1 family member LAG-1. (C) 2002 Elsevier Science (USA).