Immunoelectron microscopic localization of the M6a antigen in rat brain
Immunoelectron microscopic localization of the M6a antigen in rat brain
复制标题
大鼠脑中 M6a 抗原的免疫电镜定位
DOI：
--
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
J. Nussbaum
中科院分区：
文献类型：
--
作者：
G. Roussel;É. Trifilieff;C. Lagenaur;J. Nussbaum
单克隆抗体 M6-7 可识别天然和变性的免疫纯化 M6a 抗原，在本免疫细胞化学研究中用于在幼鼠脑中定位其相应抗原。在小脑分子层中观察到强标记，这对应于源自颗粒细胞的重度染色的轴突末端。通过电子显微镜观察，免疫沉积物仅存在于突触前膜的细胞质侧和突触小泡的膜上。相比之下，浦肯野细胞及其过程未染色。在其他几个大脑区域也发现了染色的突触，尽管频率较低。这些突触的染色模式与在小脑分子层中观察到的相似。基于其在某些神经元末梢中的有限分布及其与髓磷脂蛋白脂质的高度同源性，推测M6-7抗体揭示的M6a抗原可能参与这些结构中的特定生物学功能。
The monoclonal antibody M6-7, which recognizes both native and denatured immunopurified M6a antigen, was used in the present immunocytochemical study to localize its corresponding antigen in young rat brain. Strong labelling was observed in the cerebellar molecular layer, which corresponds to heavily stained axon terminals originating from granule cells. The immunodeposit, as observed by electron microscopy, is present only on the cytoplasmic side of the presynaptic membrane and on the membrane of synaptic vesicles. In contrast, the Purkinje cells and their processes are unstained. Stained synapses are also found, although less frequently, in several other cerebral areas. The pattern of staining at these synapses is similar to that observed in the cerebellar molecular layer. It is hypothesized, on the basis of its restricted distribution in certain neuronal endings and its high homology with myelin proteolipids, that the M6a antigen revealed by the M6-7 antibody is probably involved in a specific biological function in these structures.
二环己基碳二亚胺与髓磷脂蛋白脂质的相互作用。
DOI：
10.1073/pnas.79.3.941
发表时间：
1982
影响因子：
11.1
作者：
Lin,LF;Lees,MB
通讯作者：
Lees,MB