Genome-wide characterization of monomeric transcriptional regulators in Mycobacterium tuberculosis
Genome-wide characterization of monomeric transcriptional regulators in Mycobacterium tuberculosis
复制标题
结核分枝杆菌单体转录调节因子的全基因组特征
DOI：
10.1099/mic.0.000257
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发表时间：
2016-05-01
期刊：
影响因子：
2.8
通讯作者：
Chen, Shiyun
中科院分区：
文献类型：
--
作者：
Feng, Lipeng;Chen, Zhenkang;Chen, Shiyun
RNA聚合酶催化的基因转录受到转录调控因子的调控，转录调控因子在真核生物和原核生物的基因转录调控中都起着核心作用。在调节基因转录时，许多调节因子形成二聚体，与具有重复基序的DNA结合。然而，一些调节因子作为单体起作用，但它们的基因表达控制机制在很大程度上是未知的。在这里，我们系统地表征了结核病病原体结核分枝杆菌中的单体与二聚体调节因子。在结核分枝杆菌中注释的160个转录调节因子中，测试了154个转录调节因子，22%可能作为单体起作用，大多数被注释为假设的调节因子。值得注意的是，whb样蛋白家族的所有成员都被归类为单体。为了进一步研究单体调控机制，我们分析了这些WhiB蛋白的作用，发现大多数与主sigma因子sigma(A)相互作用，这也是RNA聚合酶全酶中的单体蛋白。综上所述，我们的研究首次在全球范围内对结核分枝杆菌中的单体调控因子进行了分类，并提出了单体调控因子通过与单体sigma因子相互作用来控制基因转录的机制。
Gene transcription catalysed by RNA polymerase is regulated by transcriptional regulators, which play central roles in the control of gene transcription in both eukaryotes and prokaryotes. In regulating gene transcription, many regulators form dimers that bind to DNA with repeated motifs. However, some regulators function as monomers, but their mechanisms of gene expression control are largely uncharacterized. Here we systematically characterized monomeric versus dimeric regulators in the tuberculosis causative agent Mycobacterium tuberculosis. Of the >160 transcriptional regulators annotated in M. tuberculosis, 154 transcriptional regulators were tested, 22% probably act as monomers and most are annotated as hypothetical regulators. Notably, all members of the WhiB-like protein family are classified as monomers. To further investigate mechanisms of monomeric regulators, we analysed the actions of these WhiB proteins and found that the majority interact with the principal sigma factor sigma(A), which is also a monomeric protein within the RNA polymerase holoenzyme. Taken together, our study for the first time globally classified monomeric regulators in M. tuberculosis and suggested a mechanism for monomeric regulators in controlling gene transcription through interacting with monomeric sigma factors.