Adaptive virus detection using filament-coupled antibodies.
Adaptive virus detection using filament-coupled antibodies.
复制标题
使用丝耦合抗体进行自适应病毒检测。
DOI：
10.1117/1.2209907
复制
发表时间：
2006
影响因子：
3.5
通讯作者：
Haselton,FrederickR
中科院分区：
文献类型：
--
作者：
Stone,GregoryP;Lin,KelvinS;Haselton,FrederickR
We recently reported the development of a filament-antibody recognition assay (FARA), in which the presence of virions in solution initiates the formation of enzyme-linked immunosorbent assay (ELISA)-like antibody complexes. The unique features of this assay are that processing is achieved by motion of a filament and that, in the presence of a virus, antibody-virus complexes are coupled to the filament at known locations. In this work, we combine the unique features of this assay with a 638-nm laser-based optical detector to enable adaptive control of virus detection. Integration of on-line fluorescence detection yields approximately a five-fold increase in signal-to-noise ratio (SNR) compared to the fluorescence detection method reported previously. A one-minute incubation with an M13K07 test virus is required to detect, andwas required to detect. In tests of the components of an adaptive strategy, avirusincubation time, followed by repositioning the filament-captured virus either within the detecting antibody chamber,or within the virus chamber, found an increase in signal roughly proportional to the cumulative residence times in these chambers. Furthermore, cumulative fluorescence signals observed for a filament-captured virus after repeated positioning of the filament within the virus chamber are similar to those observed for a single long incubation time. The unique features of the FARA-like design combined with online optical detection to direct subsequent bioprocessing steps provides new flexibility for developing adaptive molecular recognition assays.
