THE EFFECT OF SINGLE DOSES OF RADIATION ON MOUSE SPINAL-CORD
THE EFFECT OF SINGLE DOSES OF RADIATION ON MOUSE SPINAL-CORD
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DOI：
10.1016/0360-3016(92)90982-n
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发表时间：
1992-01-01
影响因子：
7
通讯作者：
WITHERS, HR
中科院分区：
文献类型：
--
作者：
LO, YC;MCBRIDE, WH;WITHERS, HR
我们使用小鼠模型研究单次剂量（12至75戈伊）放射后的脊髓损伤。 照射C3 Hf/Sed//Kam小鼠的脊髓（T9，10-L4，5），并使用四个级别的神经变化对反应进行分级。 调查结果如下：（a）在辐射后200-360天测量，轻度、部分和完全麻痹50%动物所需剂量（ED 50）分别为19.79、20.77和21.85戈伊。 (b)大多数损害是渐进的，但不一定如此;剂量高达28戈伊后，偶尔可以看到恢复。 (c)潜伏期取决于剂量和损伤程度。 在ED 50左右给药后，瘫痪发生在180至300天之间。 (d)在剂量小于35戈伊时，剂量-潜伏期关系有显著波动。 潜伏期可能不是比较该剂量范围内辐射生物效应的可靠终点。 (e)小鼠脊髓的放射敏感性与大鼠相似。 (f)组织学上，脱髓鞘是瘫痪动物的主要病变。 我们的结论是，小鼠是一个很好的动物模型，以研究辐射损伤的脊髓，至少当一个2.2厘米的长度被照射。
We have used a mouse model to study spinal cord injury following single doses (12 to 75 Gy) of radiation. The spinal cord (T9,10-L4,5) of C3Hf/Sed//Kam mice was irradiated and response graded using four levels of neurological change. Findings were: (a) the doses required to paralyze 50% of animals (ED50) were 19.79, 20.77, and 21.85 Gy for mild, partial, and complete paralysis, respectively, as measured 200-360 days after radiation. (b) Most damage was progressive but it was not necessarily so; after doses up to 28 Gy recovery was occasionally seen. (c) Latency depended on the dose and the level of damage. Following doses around the ED50, paralysis occurred between 180 to 300 days. (d) There were significant fluctuations in the dose-latency relationship at doses less than 35 Gy. Latency may be not a reliable endpoint to compare biological effects of radiation in this dose range. (e) The radiosensitivity of mouse spinal cord was similar to that reported for rats. (f) Histologically, demyelination was the dominant lesion in the paralyzed animals. We conclude that the mouse is a good animal model to study radiation damage to the spinal cord, at least when a 2.2 cm length is irradiated.