Differential expression of miRNAs in response to salt stress in maize roots
Differential expression of miRNAs in response to salt stress in maize roots
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DOI：
10.1093/aob/mcn205
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发表时间：
2009-01-01
期刊：
影响因子：
4.2
通讯作者：
Zheng, Yonglian
中科院分区：
文献类型：
--
作者：
Ding, Dong;Zhang, Lifang;Zheng, Yonglian
玉米（Zea mays）通过基因表达、代谢和生理变化对盐胁迫做出响应。这种适应是通过在转录和转录后水平调节基因表达来实现的。microRNA（miRNAs）是转录后基因表达的关键调控因子。然而，对于miRNAs在植物对非生物胁迫的响应中的作用知之甚少。使用含有Release version 10.1植物miRNAs探针（http：//microrna.sanger.ac.uk/）的定制mu paraflo（TM）微流控阵列，利用耐盐玉米自交系“NC 286”和盐敏感玉米系“黄早4”之间的miRNAs表达差异来发现盐胁迫响应miRNAs。这些miRNA在盐响应中表现出不同的活性，并且属于相同miRNA家族的miRNA表现出相同的行为。有趣的是，发现18个miRNAs仅在耐盐玉米系中表达，而25个miRNAs在盐敏感系中表现出延迟调节模式。提出了一个基因模型，揭示了miRNAs如何调控非生物胁迫相关过程和应对胁迫的基因网络，即盐响应miRNAs在转录后水平参与玉米幼苗的代谢、形态和生理适应的调控。miRNA基因型特异性表达模型可以解释玉米品系间不同的盐敏感性。
Corn (Zea mays) responds to salt stress via changes in gene expression, metabolism and physiology. This adaptation is achieved through the regulation of gene expression at the transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) have been found to act as key regulating factors of post-transcriptional gene expression. However, little is known about the role of miRNAs in plants' responses to abiotic stresses.A custom mu paraflo (TM) microfluidic array containing release version 10.1 plant miRNA probes (http://microrna.sanger.ac.uk/) was used to discover salt stress-responsive miRNAs using the differences in miRNA expression between the salt-tolerant maize inbred line 'NC286' and the salt-sensitive maize line 'Huangzao4'.miRNA microarray hybridization revealed that a total of 98 miRNAs, from 27 plant miRNA families, had significantly altered expression after salt treatment. These miRNAs displayed different activities in the salt response, and miRNAs belonging to the same miRNA family showed the same behaviour. Interestingly, 18 miRNAs were found which were only expressed in the salt-tolerant maize line, and 25 miRNAs that showed a delayed regulation pattern in the salt-sensitive line. A gene model was proposed that showed how miRNAs could regulate the abiotic stress-associated process and the gene networks coping with the stress.Salt-responsive miRNAs are involved in the regulation of metabolic, morphological and physiological adaptations of maize seedlings at the post-transcriptional level. The miRNA genotype-specific expression model might explain the distinct salt sensitivities between maize lines.