Sodium dodecyl sulfate agarose gel electropheresis and electroelution of high molecular weight human salivary mucin
Sodium dodecyl sulfate agarose gel electropheresis and electroelution of high molecular weight human salivary mucin
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DOI：
10.1007/s00784-005-0007-2
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发表时间：
2005-12-01
影响因子：
3.4
通讯作者：
Zentner, A
中科院分区：
文献类型：
--
作者：
Gabriel, M;Zentner, A
本研究旨在探讨十二烷基硫酸钠琼脂糖凝胶电泳法、电洗脱法和电渗滤法作为分离、检测和纯化高相对分子质量人唾液粘蛋白的适用性。经密度梯度超速离心法制备的全唾液和粘蛋白的SDS琼脂糖凝胶电泳图显示，分子量大于450 kDa的条带在1.6×10(6)~2×10(6)Da之间。从凝胶中电洗脱物质，然后进行电泳会得到高纯度的高分子量物质。电泳过滤是一种将低分子组分渗透到SDS聚丙烯酰胺凝胶中后，将全唾液中残留的物质收集到狭缝中的方法，但无法产生纯的高分子量物质。结论：SDS琼脂糖凝胶电泳法和电洗脱法是研究高相对分子质量唾液粘蛋白糖蛋白的合适方法。
The aim of this study was to test the applicability of sodium dodecyl sulfate (SDS) agarose gel electrophoresis, electroelution and electrophoretic filtration as methods of separation, detection, and purification of high molecular weight human salivary mucin. SDS agarose gel electrophoresis of whole saliva and mucin prepared by density gradient ultracentrifugation revealed bands with molecular weights in excess of 450 kDa and between 1.6 x 10(6) and 2 X 10(6) Da. Electroelution of material from gel and subsequent electrophoresis resulted in highly purified high molecular weight material. Electrophoretic filtration, a method of collecting the material remaining from whole saliva in the slot after penetration of low molecular weight constituents into SDS polyacrylamide gel, failed to produce pure high molecular weight material. It is concluded that SDS agarose gel electrophoresis and electroelution are suitable methods for studying high molecular weight salivary mucin glycoproteins.