A lipoprotein-cholesterol-albumin serum substitute stimulates Giardia lamblia encystation vesicle formation.
A lipoprotein-cholesterol-albumin serum substitute stimulates Giardia lamblia encystation vesicle formation.
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脂蛋白-胆固醇-白蛋白血清替代品可刺激兰氏贾第鞭毛虫包囊囊泡的形成。
DOI：
10.1111/j.1550-7408.1995.tb05917.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Gillin,FD
中科院分区：
文献类型：
--
作者：
Reiner,DS;Hetsko,ML;Gillin,FD
我们之前发现，与相关克隆C6相比，未在体外形成囊泡的贾第鞭毛虫WB A6克隆在分化早期被阻断，因为它不能有效地形成囊泡分泌囊泡（ESV）或表达囊泡抗原。我们现在报道A6在哺乳小鼠模型中正常形成ESV。因此，我们想知道我们的含血清的encystation培养基是否缺乏刺激或成分，或者含有A6对ESV形成特别敏感的抑制剂。我们发现，用脂蛋白-胆固醇溶液和牛血清白蛋白（LPC）代替牛血清，在囊化前和囊化培养基中增加了A6和C6的ESV形成。A6细胞携带ESV的比例从BS培养基的8%增加到LPC培养基的48%，而C6细胞携带ESV的比例为64%和98%。同样，A6的平均ESV/阳性细胞数从BS培养基的1.5个增加到LPC培养基的7.7个，C6的平均ESV/阳性细胞数从13.3个增加到19.7个。此外，在LPC胞浆培养基中，A6表达了单克隆GCSA‐1识别的囊壁表位。尽管A6在LPC培养基中形成的抗水囊肿增加了60倍，但囊肿的数量仅为C6的3-15%。这表明LPC可能主要影响胞内淤积的早期事件，而A6可能在胞内淤积的后期需要额外的因素。
We found previously that the A6 clone ofGiardia lambliastrain WB that did not encyst in vitro was blocked at an early stage in differentiation, as it did not form encystation secretory vesicles (ESV) efficiently or express cyst antigens, in comparison with the related clone C6. We now report that A6 formed ESV normally in the suckling mouse model. Therefore, we asked whether our serum‐containing encystation media might lack a stimulus or component or contain an inhibitor of ESV formation to which A6 was especially sensitive. We found that replacing bovine serum with a lipoprotein‐cholesterol solution and bovine serum albumin (LPC) in pre‐encystation and encystation media increased ESV formation by both A6 and C6. The % of A6 cells with ESV increased from 8% in BS medium to 48% in LPC medium, compared with 64% and 98% for C6. Similarly, the average number of ESV/positive cell increased from 1.5 in BS medium to 7.7 in LPC medium for A6, and from 13.3 to 19.7 for C6. Moreover, in LPC encystation media, A6 expressed the cyst wall epitope recognized by monoclonal GCSA‐1. Although formation of water‐resistant cysts by A6 was increased >60 fold in LPC media, the numbers of cysts remained only ∼3–15% that of C6. This suggests that LPC may primarily affect early events in encystation and that A6 may require additional factors later in encystation.