Phagocytosis of a PFOB-Nanoemulsion for 19F Magnetic Resonance Imaging: First Results in Monocytes of Patients with Stable Coronary Artery Disease and ST-Elevation Myocardial Infarction
Phagocytosis of a PFOB-Nanoemulsion for 19F Magnetic Resonance Imaging: First Results in Monocytes of Patients with Stable Coronary Artery Disease and ST-Elevation Myocardial Infarction
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DOI：
10.3390/molecules24112058
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发表时间：
2019-06-01
期刊：
影响因子：
4.6
通讯作者：
Boenner, Florian
中科院分区：
文献类型：
--
作者：
Nienhaus, Fabian;Colley, Denise;Boenner, Florian
静脉内应用全氟辛基溴纳米乳剂（PFOB-NE）的氟-19磁共振成像（F-19 MRI）已证明其在实验性疾病模型中可视化炎症过程的可行性。这种方法是基于单核细胞/巨噬细胞摄取全氟辛烷磺酸-NE颗粒的特性，从而能够在体内进行特定的细胞跟踪。然而，缺乏有关安全性（细胞功能和活力）、摄入机制以及特定疾病环境对PFOB-NE摄入的影响的信息。然而，这些信息对于解释F-19 MRI信号和可能的临床应用至关重要。为了解决这些问题，从急性ST段抬高型心肌梗死（STEMI）、稳定型冠状动脉疾病（SCAD）患者和健康志愿者中采集全血样本。将样品暴露于荧光标记的PFOB-NE，并通过流式细胞术和MRI评价颗粒摄取、细胞活力和迁移活性。我们能够表明，PFOB-NE是由人类单核细胞摄取的时间和子集依赖性的方式通过主动吞噬。单核细胞的功能（迁移、吞噬）和活力在全氟辛烷磺酸-去甲肾上腺素摄取后得以维持。与健康对照组相比，STEMI和SCAD患者的单核细胞的最大PFOB-NE摄取没有差异。总之，我们的研究为PFOB-NE在人类中用于成像目的的安全翻译提供了进一步的证据。
Fluorine-19 magnetic resonance imaging (F-19 MRI) with intravenously applied perfluorooctyl bromide-nanoemulsions (PFOB-NE) has proven its feasibility to visualize inflammatory processes in experimental disease models. This approach is based on the properties of monocytes/macrophages to ingest PFOB-NE particles enabling specific cell tracking in vivo. However, information on safety (cellular function and viability), mechanism of ingestion and impact of specific disease environment on PFOB-NE uptake is lacking. This information is, however, crucial for the interpretation of F-19 MRI signals and a possible translation to clinical application. To address these issues, whole blood samples were collected from patients with acute ST-elevation myocardial infarction (STEMI), stable coronary artery disease (SCAD) and healthy volunteers. Samples were exposed to fluorescently-labeled PFOB-NE and particle uptake, cell viability and migration activity was evaluated by flow cytometry and MRI. We were able to show that PFOB-NE is ingested by human monocytes in a time- and subset-dependent manner via active phagocytosis. Monocyte function (migration, phagocytosis) and viability was maintained after PFOB-NE uptake. Monocytes of STEMI and SCAD patients did not differ in their maximal PFOB-NE uptake compared to healthy controls. In sum, our study provides further evidence for a safe translation of PFOB-NE for imaging purposes in humans.