Versatile Nickel(II) Scaffolds as Coordination‐Induced Spin‐State Switches for 19 F Magnetic Resonance‐Based Detection
Versatile Nickel(II) Scaffolds as Coordination‐Induced Spin‐State Switches for 19 F Magnetic Resonance‐Based Detection
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多功能镍 (II) 支架作为协调诱导自旋状态开关，用于基于 19 F 磁共振的检测
DOI：
10.1002/anie.202010587
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Que, Emily L.
中科院分区：
文献类型：
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作者：
Xie, Da;Yu, Meng;Xie, Zhu‐Lin;Kadakia, Rahul T.;Chung, Chris;Ohman, Lauren E.;Javanmardi, Kamyab;Que, Emily L.
基于19 F磁共振（MR）的检测与精心设计的无机系统相结合，在生物学研究中显示出希望。报道了两种基于从低自旋（S=0）到高自旋（S=1）Ni 2+转换的19 F MR传感新机制的概念验证无机探针。分别通过光或β-半乳糖苷酶活化抗磁性NiL 1和NiL 2，将其转化为顺磁性NiL 0，其显示单个19 F NMR峰偏移>35 ppm，并具有加速的弛豫速率。 这种自旋状态开关对于使用19 F MR光谱和成像来感测活细胞中的光或酶表达是有效的，所述19 F MR光谱和成像基于化学位移和弛豫时间区分信号。这种通用的无机支架具有开发试剂的潜力，可以检测从离子到酶的分析物，为基于19 F MR的生物传感开辟了多种可能性。
19F magnetic resonance (MR) based detection coupled with well‐designed inorganic systems shows promise in biological investigations. Two proof‐of‐concept inorganic probes that exploit a novel mechanism for19F MR sensing based on converting from low‐spin (S=0) to high‐spin (S=1) Ni2+are reported. Activation of diamagneticNiL1andNiL2by light or β‐galactosidase, respectively, converts them into paramagneticNiL0, which displays a single19F NMR peak shifted by >35 ppm with accelerated relaxation rates. This spin‐state switch is effective for sensing light or enzyme expression in live cells using19F MR spectroscopy and imaging that differentiate signals based on chemical shift and relaxation times. This general inorganic scaffold has potential for developing agents that can sense analytes ranging from ions to enzymes, opening up diverse possibilities for19F MR based biosensing.