Methanogenesis versus electrogenesis: Morphological and phylogenetic comparisons of microbial communities
Methanogenesis versus electrogenesis: Morphological and phylogenetic comparisons of microbial communities
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DOI：
10.1271/bbb.70179
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发表时间：
2008-02-01
影响因子：
1.6
通讯作者：
Watanabe, Kazuya
中科院分区：
文献类型：
--
作者：
Ishii, Shun'ichi;Hotta, Yasuaki;Watanabe, Kazuya
制备了两种H型微生物燃料电池。厌氧室接种水稻田土壤和饲料纤维素作为能源。在一个反应器中，阳极和阴极用导线连接（闭路，CC），而它们在另一个反应器中不连接（开路，OC）。OC反应堆活跃地产生甲烷。然而，在CC反应器中，持续产生0.2至0.3mA的电流，甲烷的产生几乎被完全抑制。电子显微镜显示，杆状细胞与长prosthecae样的细丝，特别是丰富的CC反应器。16S rRNA基因克隆库的比较显示完全不同的系统发育组成的CC和OC社区，根瘤菌，脱硫弧菌，Ethanoligenens相关的根瘤菌型，特别是丰富的CC社区。结果表明，产电导致富集的独特的微生物种群和抑制甲烷从纤维素。
Two H-type microbial fuel cells were prepared. The anaerobic chambers were inoculated with rice paddy field soil and fed cellulose as an energy source. In one reactor, the anode and cathode were connected with a wire (closed circuit, CC), while they were not connected in the other reactor (open circuit, OC). The OC reactor actively produced methane. In the CC reactor, however, an electric current of 0.2 to 0.3mA was constantly generated, and methane production was almost completely suppressed. Electron microscopy revealed that rod-shaped cells with long prosthecae-like filaments were specifically enriched in the CC reactor. Comparisons of 16S rRNA gene clone libraries revealed entirely different phylogenetic compositions in the CC and OC communities; phylotypes related to Rhizobiceae, Desulfovibrio, and Ethanoligenens were specifically enriched in the CC community. The results indicate that electrogenesis resulted in the enrichment of distinctive microbial populations and suppressed methanogenesis from cellulose.