Thyroid hormones alter estrous cyclicity and antioxidative status in the ovaries of rats
Thyroid hormones alter estrous cyclicity and antioxidative status in the ovaries of rats
复制标题
甲状腺激素改变大鼠卵巢的动情周期和抗氧化状态
DOI：
10.1111/asj.12950
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发表时间：
2018-03-01
影响因子：
2
通讯作者：
Shi, Fangxiong
中科院分区：
文献类型：
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作者：
Wei, Quanwei;Fedail, Jaafar Sulieman;Shi, Fangxiong
为了扩大我们对甲状腺激素对女性生殖的作用的理解，我们诱导低T和高T大鼠模型，以调查甲状腺激素对动情周期的作用，以及大鼠卵巢的抗氧化状态。在本研究中，我们的数据显示，甲状腺功能减退症（hypo-T）和甲状腺功能亢进症（hyper-T）导致体重和卵巢重量显着降低，阴道开放日延迟。对于高T，甲状腺素（T4），三碘甲状腺原氨酸（T3），孕酮（P4）和卵泡刺激素（FSH）显着增加，而雌二醇（E2）和黄体生成素（LH）显着降低。低T大鼠血清总T4、T3、E2、P4、FSH和LH水平显著升高，E2和LH水平显著降低。在卵巢形态学方面，高T组和低T组次级卵泡和有腔卵泡数量均显著减少，闭锁的有腔卵泡增多，黄体减少。高T和低T处理均显著降低卵巢甲状腺激素受体1的表达。低T显著降低一氧化氮（NO）、总NO合酶（tNOS）、诱导型NOS和组成型NOS活性，而高T则使其升高。对于抗氧化指标，低T和高T处理显着增加了丙二醛（MDA）含量。谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性在低T组显著降低，而在高T组显著升高。高T组总超氧化物歧化酶（T-SOD）活性显著升高。总之，甲状腺激素改变大鼠的动情周期和卵巢的抗氧化状态可能通过NOS信号通路起作用。
To expand our understanding of the roles of thyroid hormones on female reproduction, we induced hypo- and hyper-T rat models to investigate the roles of thyroid hormones on estrous cyclicity, as well as the antioxidative status in the ovaries of rats. In the current study, our data show that hypothyroidism (hypo-T) and hyperthyroidism (hyper-T) led to significantly reduced body weights and ovarain weights and delayed vaginal opening day. For hyper-T, thyroxine (T4), tri-iodothyronine (T3), progesterone (P4) and follicle-stimulating hormone (FSH) were significantly increased, while estradiol (E2) and luteinizing hormone (LH) were significantly decreased. For hypo-T rats, serum levels of total T4 and T3, E2, P4, FSH and LH were significantly increased, while concentrations of E2 and LH were significantly decreased. For ovary morphology, the numbers of secondary and antral follicles were significantly decreased with more atretic antral follicles and less corpora lutea in both hyper- and hypo-T groups. Both hyper-T and hypo-T treatment significantly decreased the expressions of thyroid hormone receptor 1 in the ovary. Hypo-T significantly reduced nitric oxide (NO), total NO synthase (tNOS), inducible NOS and constitutive NOS activities, but hyper-T increased them. For antioxidative parameters, hypo-T and hyper-T treatment significantly increased malondialdehyde (MDA) contents. The activities of both glutathione peroxidase (GSH-Px) and catalase (CAT) significantly decreased in the hypo-T group but increased in the hyper-T group. Total superoxide dismutase (T-SOD) activity was significantly increased in the hyper-T group. In summary, thyroid hormones alter estrous cyclicity and antioxidative status in the ovary of the rat may act through the NOS signaling pathway.