Effect of antler extract on corticosteroid-induced avascular necrosis of the femoral head in rats
Effect of antler extract on corticosteroid-induced avascular necrosis of the femoral head in rats
复制标题
鹿茸提取物对皮质类固醇所致大鼠股骨头缺血性坏死的影响
DOI：
10.1016/j.jep.2009.09.036
复制
发表时间：
2010-01-08
影响因子：
5.4
通讯作者：
Yu, Gongchang
中科院分区：
文献类型：
--
作者：
Shi, Bin;Li, Gang;Yu, Gongchang
民族药理意义：鹿茸是东医传统的补益名药，具有强骨活性，可有效治疗骨病。目的：研究鹿茸提取物对激素性股骨头缺血坏死的治疗作用。材料与方法：采用大鼠臀部注射地塞米松50 mg/kg，每周2次，共6周，建立大鼠股骨头缺血性坏死模型。鹿茸提取物200 mg/kg、400 mg/kg、800 mg/kg灌胃1次/d，连续60d。测定血清羟脯氨酸和氨基己糖浓度，并进行股骨头超微结构检查。结果：鹿茸提取液作用后，地塞米松诱导的成骨细胞坏死程度明显减轻，羟脯氨酸含量显著降低，氨基己糖含量和氨基己糖/羟脯氨酸比值显著升高。鹿茸提取液含药血清通过调节细胞周期进程促进成骨细胞增殖。结论：鹿茸提取液通过促进成骨细胞增殖对股骨头坏死有积极的治疗作用。爱思唯尔爱尔兰有限公司出版。
Ethnopharmacological relevance: Deer antler, traditionally used as a tonic and valuable drug in oriental medicine, has been considered to possess bone-strengthening activity and effectively used in bone diseases therapy.Aim of the study: The present study was designed to investigate therapeutic effect of antler extract on avascular necrosis of the femoral head (ANFH) induced by corticosteroids in rats.Materials and methods: Rats were intragluteally injected with dexamethasone at 50 mg/kg twice per week for 6 weeks to induce ANFH. Then the rats were treated with antler extract by oral gavage at 200 mg/kg, 400 mg/kg and 800 mg/kg once per day for 60 days. The concentration of hydroxyproline and hexosamine in serum was measured and the ultrastructure of femoral head was examined. In vitro, effect of the drug-containing serum of antler extract on proliferation and differentiation of primary osteoblasts were investigated by MIT assay, ALP activity assay and cell cycle analysis.Results: After treatment with antler extract, the degree of necrosis induced by dexamethasone was significantly reduced, hydroxyproline was significantly decreased, and hexosamine and the ratio of hexosamine/hydroxyproline were significantly increased. The drug-containing serum of antler extract promotes osteoblastic proliferation through regulation of cell cycle progression.Conclusions: The results suggest that antler extract has a positive curative effect on ANFH by promoting osteoblastic proliferation. Published by Elsevier Ireland Ltd.