How a cyanobacterium tells time.

How a cyanobacterium tells time.
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DOI:
10.1016/j.mib.2008.10.003
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发表时间:
2008-12
影响因子:
5.4
通讯作者:
Golden, Susan S.
Golden, Susan S.
中科院分区:
生物学2区
文献类型:
--
作者:
Dong, Guogang;Golden, Susan S.

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蓝细菌细长聚球藻在由 KaiA、KaiB 和 KaiC 三种蛋白质组成的振荡器上构建了一个生物钟,它可以在体外重现 KaiC 磷酸化的昼夜节律。所有三种蛋白质的分子结构都是已知的,KaiC 的磷酸化步骤、三种 Kai 蛋白质之间的相互作用动力学以及 KaiC 的弱 ATP 酶活性都已被表征。氧化还原敏感蛋白的输入途径利用光合作用功能将光/暗信息传递给振荡器,知名家族的信号转导蛋白将时间信息广播到基因组,其中转录的全局变化和染色体的压缩受到时钟调节。
The cyanobacterium Synechococcus elongatus builds a circadian clock on an oscillator comprised of three proteins, KaiA, KaiB, and KaiC, which can recapitulate a circadian rhythm of KaiC phosphorylation in vitro. The molecular structures of all three proteins are known, and the phosphorylation steps of KaiC, the interaction dynamics among the three Kai proteins, and a weak ATPase activity of KaiC have all been characterized. An input pathway of redox-sensitive proteins uses photosynthetic function to relay light/dark information to the oscillator, and signal transduction proteins of well-known families broadcast temporal information to the genome, where global changes in transcription and a compaction of the chromosome are clock regulated.
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