Regular cellular distribution of plasmids by oscillating and filament-forming ParA ATPase of plasmid pB171
Regular cellular distribution of plasmids by oscillating and filament-forming ParA ATPase of plasmid pB171
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DOI：
10.1111/j.1365-2958.2006.05322.x
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发表时间：
2006-09-01
影响因子：
3.6
通讯作者：
Gerdes, Kenn
中科院分区：
文献类型：
--
作者：
Ebersbach, Gitte;Ringgaard, Simon;Gerdes, Kenn
细菌的类着丝粒基因座在细胞分裂前将质粒分离到子代细胞中。来自质粒pB171的par2基因座的帕拉ATP酶（MinD同源物）在类核上形成振荡螺旋结构。在这里，我们表明，par2分布质粒灶定期沿着细胞的长度，即使在细胞中有许多质粒。在体外，帕拉A结合ATP和ADP，并具有协同ATP酶活性。此外，帕拉形成ATP依赖的细丝和电缆，这表明帕拉可以提供所观察到的质粒的规则分布的机械力。在细菌双杂交试验中，帕拉和ParB相互作用，但不与FtsZ、其他八种必需的细胞分裂蛋白或MreB肌动蛋白相互作用。基于这些观察，我们提出了一个简单的模型，如何振荡的帕拉丝可以调解定期细胞分布的质粒。该模型的功能没有分区特异性宿主细胞受体的参与，因此与分区位点可以在异源宿主生物体中发挥作用的惊人观察结果一致。
Centromere-like loci from bacteria segregate plasmids to progeny cells before cell division. The ParA ATPase (a MinD homologue) of the par2 locus from plasmid pB171 forms oscillating helical structures over the nucleoid. Here we show that par2 distributes plasmid foci regularly along the length of the cell even in cells with many plasmids. In vitro, ParA binds ATP and ADP and has a cooperative ATPase activity. Moreover, ParA forms ATP-dependent filaments and cables, suggesting that ParA can provide the mechanical force for the observed regular distribution of plasmids. ParA and ParB interact with each other in a bacterial two-hybrid assay but do not interact with FtsZ, eight other essential cell division proteins or MreB actin. Based on these observations, we propose a simple model for how oscillating ParA filaments can mediate regular cellular distribution of plasmids. The model functions without the involvement of partition-specific host cell receptors and is thus consistent with the striking observation that partition loci can function in heterologous host organisms.