LYSIS AND VIABILITY OF CULTURED MAMMALIAN CELLS EXPOSED TO 1 MEGAHERTZ ULTRASOUND
LYSIS AND VIABILITY OF CULTURED MAMMALIAN CELLS EXPOSED TO 1 MEGAHERTZ ULTRASOUND
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DOI：
10.1016/0301-5629(77)90117-x
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发表时间：
1977-01-01
影响因子：
2.9
通讯作者：
CARSTENSEN E L
中科院分区：
文献类型：
--
作者：
KAUFMAN G E;MILLER M W;CARSTENSEN E L
HeLa [人宫颈癌] 和 CHO [中国仓鼠卵巢] 细胞暴露于强度高达 30 W/cm2 的 1 MHz 超声波下 1-15 分钟。细胞裂解的阈值约为 1 W/cm2，最大效果为 10 W/cm2。通过台盼蓝排除和集落形成能力测定，在完整细胞中，活力下降；强度与响应曲线与细胞裂解的曲线相似。对于剩余的完整和活细胞来说，增殖率下降并且巨细胞发生率增加。
HeLa [human cervical cancer] and CHO [Chinese hamster ovary] cells were exposed for 1-15 min to 1 MHz ultrasound at intensities up to 30 W/cm2. The threshold for cell lysis was approximately 1 W/cm2, with the maximum effect at 10 W/cm2. Among the intact cells there was a decreased viability as determined both by trypan blue exclusion and by colony-forming ability; the intensity vs. response curve was similar to that for cell lysis. A decrease in proliferation rate and an increased incidence of giant cells occur for the remaining intact and viable cells.