DEVELOPMENT OF GENETIC LINKAGE MAP OF LEAF HAIRINESS IN GOSSYPIUM HIRSUTUM (COTTON) USING MOLECULAR MARKERS
DEVELOPMENT OF GENETIC LINKAGE MAP OF LEAF HAIRINESS IN GOSSYPIUM HIRSUTUM (COTTON) USING MOLECULAR MARKERS
复制标题
DOI：
--
复制
发表时间：
2009
影响因子：
1.2
通讯作者：
I. Ali;A. Kausar;Mehboob-ur-Rehman;Y. Zafar;Muhammad A. Asif;M. Ashraf;S. Riaz;S. Zafar;A. Wahid;Sobia Maqsood;M. Niaz;S. Q. Abbas
中科院分区：
文献类型：
--
作者：
I. Ali;A. Kausar;Mehboob-ur-Rehman;Y. Zafar;Muhammad A. Asif;M. Ashraf;S. Riaz;S. Zafar;A. Wahid;Sobia Maqsood;M. Niaz;S. Q. Abbas
棉花（Gossypium hirsutum L.）基因型Rajhans（具柔毛）与FH-634（无毛）杂交，并产生F2和F3分离群体。利用RAPD和SSR技术，对400个RAPD引物和54个SSR引物进行了与毛羽性状连锁的DNA标记的筛选。在分析之前，优化PCR条件。8个RAPD引物OPD-19640、OPF-11630、OPG-06980、OPG-17500、OPH-131100、OPN-14890、OPO-11920、OPO-141200在重复反应中一致地检测到。SSR引物JESPR-154在有毛群体中扩增出150 bp的DNA片段。利用多态性DNA标记构建连锁图谱。利用RAPD和SSR标记构建的遗传连锁图谱表明，棉花叶毛性状存在遗传，可用于提高棉花抗虫性。
Cotton (Gossypium hirsutum L.) genotype Rajhans (pilose) was crossed with FH-634 (glabrous) and developed F2 and F3 segregating populations. PCR based techniques RAPD and SSR were used to determine DNA markers linked with the trait of hairiness using 400 RAPD and 54 SSR primers. Prior to analysis, PCR conditions were optimized. Eight RAPD primer markers OPD19640, OPF-11630, OPG-06980, OPG-17500, OPH-131100, OPN-14890, OPO-11920, OPO-141200 were consistently detected in duplicate reactions. One SSR primer pair, JESPR-154 amplified a 150 bp DNA fragment in hairiness population. Polymorphic DNA markers were used to develop linkage map. Construction of genetic linkage map using RAPD and SSR markers revealed that the inheritance for leaf hairiness exists, which can be used to improve insect resistance in cotton.