LYTIC ACTIVITY OF MONOMERIC AND OLIGOMERIC MELITTIN
LYTIC ACTIVITY OF MONOMERIC AND OLIGOMERIC MELITTIN
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DOI：
10.1016/0005-2736(83)90473-x
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
TATHAM, AS
中科院分区：
文献类型：
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作者：
HIDER, RC;KHADER, F;TATHAM, AS
监测由高磷酸盐抗衡离子浓度诱导的蜂毒肽和蜂毒肽四聚体的溶血活性。单体蜂毒肽完全溶解；四聚体蜂毒素缺乏这种活性。在蜂毒肽完全四聚体的条件下，尝试使用一系列不同链长的双官能酰亚胺酯来共价交联天然四聚体。交联寡聚物在蜂毒肽被证明缺乏这种活性的条件下完全裂解。这一发现连同分子量测定和圆二色性研究表明，交联蜂毒肽与天然四聚体有很大不同。含蜂毒肽溶液的溶血活性与单体蜂毒肽的浓度有关。降低的介电常数(ε)对蜂毒肽及其衍生物的聚集行为的影响有利于单体蜂毒肽。
The hemolytic activities of melittin and melittin tetramer as induced by high phosphate counterion concentration, were monitored. Monomeric melittin was fully lytic; tetrameric melittin lacked such activity. Under conditions where melittin was fully tetrameric attempts were made to covalently cross-link the native tetramer using a series of different chain length bifunctional imido esters. The cross-linked oligomers were fully lytic under conditions where melittin was demonstrated to lack such activity. This finding, together with MW determinations and circular dichroism studies, indicated that the cross-linked melittin was quite different from the native tetramer. The hemolytic activity of melittin-containing solutions was related to the concentration of monomeric melittin. The effect of reduced dielectric constant (.epsilon.) on the aggregation behavior of melittin and its derivatives was favored monomeric melittin.