Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering.
Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering.
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用于血管组织工程的电纺明胶/PCL 和胶原/PLCL 支架
DOI：
10.2147/ijn.s61375
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发表时间：
2014
影响因子：
8
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Fu W;Liu Z;Feng B;Hu R;He X;Wang H;Yin M;Huang H;Zhang H;Wang W
利用天然和合成聚合物的组合制备的电纺杂化纳米纤维在心血管组织工程中已被广泛研究。本研究成功制备了明胶/聚己内酯（PCL）和胶原/聚（L-乳酸-co-ε-己内酯）（PLCL）静电纺丝支架。扫描电子显微镜显示，两种膜的纤维光滑均匀。水接触角测量进一步证明两种支架都是亲水性的。为了确定细胞在支架上的附着和迁移，将两种混合支架与人脐动脉平滑肌细胞一起接种。扫描电子显微镜和MTT法显示，细胞在两种杂化支架上生长增殖良好。移植支架的大体观察显示，工程化胶原/PLCL支架比明胶/PCL支架更光滑、更明亮。苏木精-伊红染色显示胶原/PLCL电纺膜构建的工程血管形成了相对均一的血管样组织。有趣的是，工程化胶原/PLCL支架的杨氏模量大于明胶/PCL支架。总之，这些结果表明，具有良好的机械和生物学特性的纳米纤维胶原/PLCL膜可能是血管组织工程的理想支架。
Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in cardiovascular tissue engineering. In this study, electrospun gelatin/polycaprolactone (PCL) and collagen/poly(l-lactic acid-co-ε-caprolactone) (PLCL) scaffolds were successfully produced. Scanning electron micrographs showed that fibers of both membranes were smooth and homogeneous. Water contact angle measurements further demonstrated that both scaffolds were hydrophilic. To determine cell attachment and migration on the scaffolds, both hybrid scaffolds were seeded with human umbilical arterial smooth muscle cells. Scanning electron micrographs and MTT assays showed that the cells grew and proliferated well on both hybrid scaffolds. Gross observation of the transplanted scaffolds revealed that the engineered collagen/PLCL scaffolds were smoother and brighter than the gelatin/PCL scaffolds. Hematoxylin and eosin staining showed that the engineered blood vessels constructed by collagen/PLCL electrospun membranes formed relatively homogenous vessel-like tissues. Interestingly, Young’s modulus for the engineered collagen/PLCL scaffolds was greater than for the gelatin/PCL scaffolds. Together, these results indicate that nanofibrous collagen/PLCL membranes with favorable mechanical and biological properties may be a desirable scaffold for vascular tissue engineering.