SZ95 sebocytes induce epidermal melanocyte dendricity and proliferation in vitro
SZ95 sebocytes induce epidermal melanocyte dendricity and proliferation in vitro
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DOI：
10.1111/j.1600-0625.2012.01468.x
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发表时间：
2012-05-01
影响因子：
3.6
通讯作者：
Zouboulis, Christos C.
中科院分区：
文献类型：
--
作者：
Abdel-Naser, Mohamed Badawy;Seltmann, Holger;Zouboulis, Christos C.
皮脂腺细胞对黑素细胞（HMel）的调节作用尚不清楚。在这项研究中，SZ 95皮脂腺细胞与HMel共培养，无论是直接细胞接触还是与插入物中的SZ 95皮脂腺细胞共培养，都导致表皮HMel变平，表面积增加，并形成多个小树突。只有在高Ca ~（2+）水平和直接细胞接触共培养时，HMel树突才显著长，并优先靶向和附着于SZ 95皮脂腺细胞。同样地，当与第0天相比时，仅高Ca 2 + SZ 95皮脂细胞条件培养基在孵育的第9天和第12天以时间依赖性方式刺激HMel增殖（142.9%，P < 0.01）和（179.2%，P < 0.0001）。与低Ca ~（2+）SZ_（95）细胞相比，高Ca ~（2+）SZ_（95）细胞培养第6天和第9天黑色素含量显著降低（P < 0.01和P < 0.05）。这些结果表明，皮脂腺细胞也调节HMel功能，并可能有助于在皮脂腺丰富的身体区域的皮肤颜色。
The regulatory effects of sebocytes on melanocytes (HMel) are unknown. In this study, SZ95 sebocytes co-cultured with HMel, whether in direct cell contact or with SZ95 sebocytes in inserts, resulted in epidermal HMel flattening with increase in surface area and multiple small dendrites formation. Only in high Ca2+ level and direct cell contact co-culture, the HMel dendrites were remarkably long and preferentially targeted and attached to SZ95 sebocytes. Likewise, only high Ca2+ SZ95 sebocyte conditioned medium stimulated HMel proliferation in a time-dependent manner at days 9 (142.9%, P < 0.01) and 12 (179.2%, P < 0.0001) of incubation when compared with day 0. In contrast, melanin contents significantly decreased on incubation with high Ca2+ SZ95 sebocytes in comparison with low Ca2+ SZ95 sebocytes at days 6 (P < 0.01) and 9 (P < 0.05) of incubation. These results denote that sebocytes also modulate HMel functions and may contribute to skin colour in sebaceous glands-rich body regions.