Cationic liposome-mediated gene delivery: Biophysical study and mechanism of internalization
Cationic liposome-mediated gene delivery: Biophysical study and mechanism of internalization
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DOI：
10.1016/s0003-9861(02)00725-7
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发表时间：
2003-02-15
影响因子：
3.9
通讯作者：
Kiwada, H
中科院分区：
文献类型：
--
作者：
Almofti, MR;Harashima, H;Kiwada, H
为了确定影响阳离子脂质体介导的基因递送效率的因素，我们研究了不同（+/-）电荷比的脂质体/DNA复合物（lipoplexes）的生物物理特性、通过原子力显微镜（AFM）监测的它们的结构以及它们内化到细胞中的机制之间的关系。在加入DNA后，通过AFM观察到脂质体的粒径和zeta电位以及它们的结构发生了显著变化，这取决于（+/-）电荷比。AFM图像显示，脂质复合物形成广泛融合和明显均匀的脂质颗粒封装DNA。发现脂复合物通过内吞途径内化细胞。发现脂质复合物-细胞融合主要发生在质膜水平;然而，发现这种脂质复合物-细胞膜融合对于大颗粒的摄取是必需的。一个新的观点为大的lipoplex颗粒内化到细胞质进行了讨论。(C)2002年爱思唯尔科学（美国）。All rights reserved.
To identify factors affecting cationic liposome-mediated gene delivery efficiency, we studied the relationship between the biophysical characteristics of liposome/DNA complexes (lipoplexes) at different (+/-) charge ratios, their structures as monitored by atomic force microscopy (AFM), and their mechanism(s) of internalization into the cells. Significant changes were observed in the particle size and zeta potential of liposomes and their structures assessed by AFM upon addition of DNA, which depended on (+/-) charge ratios. AFM images showed that lipoplexes were formed from extensively fused and apparently homogeneous lipid particles encapsulating DNA. Lipoplexes were found to internalize the cells through the endocytosis pathway. Lipoplex-cell fusion was found to occur mainly at the plasma membrane level; however, this lipoplex-cell membrane fusion was found to be essential for the uptake of the large particles. A new perspective for the internalization of large lipoplex particles into cytoplasm is discussed. (C) 2002 Elsevier Science (USA). All rights reserved.