Changing pattern of classical swine fever virus genogroup from classical 1.1 to emerging 2.2 in India
Changing pattern of classical swine fever virus genogroup from classical 1.1 to emerging 2.2 in India
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DOI：
10.1007/s13337-017-0368-6
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发表时间：
2017-03
期刊：
影响因子：
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通讯作者：
V. Singh;K. Rajak;Ravi Kumar;S. Raut;A. Saxena;D. Muthuchelvan;R. Singh;A. B. Pandey
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文献类型：
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作者：
V. Singh;K. Rajak;Ravi Kumar;S. Raut;A. Saxena;D. Muthuchelvan;R. Singh;A. B. Pandey
猪瘟是猪最重要的病毒性疾病之一，具有很高的经济影响。猪瘟病毒(CSFV)是黄毒科猪瘟病毒属的成员，在结构和抗原性上与该属其他成员有亲缘关系。病毒株和基因类型的鉴定可以方便地用于追踪病毒传播的来源和模式，这在很大程度上是控制策略的一部分。在本研究中，我们对来自全国各地的24份猪瘟病毒临床标本的5‘非翻译区和E2区进行了部分测序和分析。其中，5‘非编码区和E2区的序列比对在核苷酸水平上分别有96.7-100%和94.7-100%的同源性。研究中的样本显示与印度报告的其他CSFV分离株非常相似。在系统发育分析中，所有的野外样本都聚为2.2亚组。因此，这项研究为2.2亚组CSFV的出现提供了进一步的系统发育证据，取代了印度主要的1.1亚组病毒。由于印度关于CSFV的分子流行病学信息很少，因此有必要生成此类流行病学数据，以帮助理解印度全国范围内的疾病控制计划，以维持印度养猪业的发展。
Classical swine fever (CSF) is one of the most important viral diseases of pigs with high economic impact. The causative agent, Classical swine fever virus (CSFV) is a member of genusPestivirusin familyFlaviviredaeand is structurally and antigenically related to other members of the genus. The identification of virus strains and genotypes can conveniently be used to trace the origin and patterns of virus spread, which contribut substantially in control strategies. In the present study, we have partially sequenced and analysed the 5′ untranslated region (UTR) and E2 regions of CSFV clinical samples (n= 24) from various parts of the country. Among the samples, the sequence alignment of 5′UTR and E2 regions revealed 96.7–100 and 94.7–100% identities at the nucleotide level, respectively. The samples under study showed the close resemblance to the other CSFV isolates reported in India. In phylogenetic analysis, all the field samples were clustered in subgroup 2.2. Thus the study presents a further phylogenetic evidence for the emergence of subgroup 2.2 CSFV replacing the predominant subgroup 1.1 viruses in India. As the information regarding the molecular epidemiology the CSFV in india is very little, generation of such epidemiological data is warranted to help in comprehensing the nationwide disease control program to sustain the growth of pig industry in India.