A Natural Mouse Model for Neisseria Persistent Colonization.
A Natural Mouse Model for Neisseria Persistent Colonization.
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DOI：
10.1007/978-1-4939-9496-0_23
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发表时间：
2019
影响因子：
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通讯作者：
Katherine A. Rhodes;Mancheong Ma;Magdalene So
中科院分区：
文献类型：
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作者：
Katherine A. Rhodes;Mancheong Ma;Magdalene So
我们开发了一种自然小鼠模型来研究共生奈瑟氏菌的持续定植。该系统将普通实验室小鼠与肌肉奈瑟氏菌(Nmus)耦合，Nmus是野生小鼠口腔和肠道的共生体，Nmus与其自然宿主配对绕过了宿主限制障碍，这些限制阻碍了以前对NeisSerain活体行为的研究。该模型首次允许对无症状定植的宿主和近亲决定因素进行剖析。接种过程是非侵入性的，对NMUS定植的敏感性因宿主遗传背景而异。在定植的小鼠中，接种后一年都能检测到细菌负荷，使其成为研究持久性的理想模型。由于NMUS编码了几个淋球菌(和脑膜炎奈瑟菌)宿主相互作用因子，该系统可以用来查询这些共同持有的基因和因子的体内功能。NMUS还编码了许多致病奈瑟拉疫苗靶点，包括多糖胶囊，使该模型有可能用于疫苗开发。NMUS基因操作的简便性增加了这类研究的可行性。
We have developed a natural mouse model to study persistent colonization by commensalNeisseria. The system couples the ordinary lab mouse withNeisseria musculi(Nmus), a commensal in the oral cavity and gut of the wild mouse,Mus musculus.The pairing of Nmus with its natural reservoir circumvents host restriction barriers that have impeded previous studies ofNeisseriain vivo behavior. The model allows, for the first time, for the dissection of host and neisserial determinants of asymptomatic colonization. Inoculation procedures are noninvasive and susceptibility to Nmus colonization varies with host genetic background. In colonized mice, bacterial burdens are detectable up to 1-year post inoculation, making it an ideal model for the study of persistence. As Nmus encodes severalNeisseria gonorrhoeae(andNeisseria meningitidis) host interaction factors, the system can be used to query the in vivo functions of these commonly held genes and factors. Nmus also encodes many pathogenicNeisseriavaccine targets including a polysaccharide capsule, making the model potentially useful for vaccine development. The ease of genetic manipulation of Nmus enhances the feasibility of such studies.