Rapid and dynamic alternative splicing impacts the Arabidopsis cold response transcriptome
Rapid and dynamic alternative splicing impacts the Arabidopsis cold response transcriptome
复制标题
快速动态的选择性剪接影响拟南芥冷反应转录组
DOI：
10.1101/251876
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Calixto C
中科院分区：
文献类型：
--
作者：
Calixto C
植物通过基因表达重编程来适应和适应不断变化的环境条件，但选择性剪接（AS）在胁迫反应中的作用机制尚不清楚。暴露于寒冷的拟南芥植物的时间序列的rna测序决定了显著AS变化的时间。这表明，在温度降低的最初几个小时内，转录和AS活性的同步波发生，并且在整个寒冷过程中进一步发生AS反应，这表明了大规模和快速的AS反应。特别是，数百个基因由于对寒冷的反应而迅速发生AS而表现出表达变化（“早期AS”基因）；其中包括许多新的冷响应转录因子和剪接因子/RNA结合蛋白仅由AS调节。其中一些基因的AS的速度和对微小温度变化的敏感性表明，通过AS途径的微调表达有助于表达的热可塑性。四种早期AS剪接调节基因先前已被证明是抗冻性和适应性所必需的；我们提供了第五个基因的证据，U2B " -LIKE。这些因素可能驱动下游基因的AS级联反应，与转录一起调节转录组重编程，共同控制植物对低温的生理和生存反应。
Plants have adapted to tolerate and survive constantly changing environmental conditions by reprogramming gene expression The dynamics of the contribution of alternative splicing (AS) to stress responses are unknown. RNA-sequencing of a time-series ofArabidopsis thalianaplants exposed to cold determines the timing of significant AS changes. This shows a massive and rapid AS response with coincident waves of transcriptional and AS activity occurring in the first few hours of temperature reduction and further AS throughout the cold. In particular, hundreds of genes showed changes in expression due to rapidly occurring AS in response to cold (“early AS” genes); these included numerous novel cold-responsive transcription factors and splicing factors/RNA binding proteins regulated only by AS. The speed and sensitivity to small temperature changes of AS of some of these genes suggest that fine-tuning expression via AS pathways contributes to the thermo-plasticity of expression. Four early AS splicing regulatory genes have been shown previously to be required for freezing tolerance and acclimation; we provide evidence of a fifth gene,U2B”-LIKE. Such factors likely drive cascades of AS of downstream genes that, alongside transcription, modulate transcriptome reprogramming that together govern the physiological and survival responses of plants to low temperature.