Chromosomal location of the Bacillus subtilis aspartokinase II gene and nucleotide sequence of the adjacent genes homologous to uvrC and trx of Escherichia coli.
Chromosomal location of the Bacillus subtilis aspartokinase II gene and nucleotide sequence of the adjacent genes homologous to uvrC and trx of Escherichia coli.
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枯草芽孢杆菌天冬氨酸激酶II基因的染色体位置及与大肠杆菌uvrC和trx同源的相邻基因的核苷酸序列。
DOI：
10.1099/00221287-135-11-2931
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Paulus,H
中科院分区：
文献类型：
--
作者：
Chen,NY;Zhang,JJ;Paulus,H
枯草芽孢杆菌的天冬氨酸激酶II（ask）操纵子由两个相重叠的基因组成，这些基因编码天冬氨酸激酶赖氨酸敏感同工酶的两个亚基（ATP:l-天冬氨酸4-磷酸转移酶，EC 2.7.2.4）。askoperon的转导定位，通过重组插入atmarker使其失活，表明在leuandarog之间的染色体位置（约253°）。因此，asky离ecb较远，这就排除了aec可能是天冬氨酸激酶II结构基因的位点，但与aec和uvrb接近。对askoperon上游的2kb DNA片段的核苷酸序列进行了测定，发现包含两个开放阅读框。推断的远端阅读框氨基酸序列与大肠杆菌氧化还氧蛋白具有广泛的同源性，而与askpromoter重叠的近端阅读框氨基酸序列与推断的theE产物具有同源性。杆菌uvrCgene。近端开放阅读框的插入突变导致丝裂霉素敏感表型，与DNA修复中的作用一致。结合M. Petricek， L. Rutberg和L. Hederstedt [FEMS微生物学快报61,85-88]的数据，我们的结果确定了b的8.8 kb片段的核苷酸序列。subtilis染色体253°附近，基因顺序如下：trx-uvrB-ask-orfX-sdhC-sdhA-sdhB-orfY-gerE。
The aspartokinase II(ask) operon ofBacillus subtilisconsists of two in-phase overlapping genes that encode the two subunits of the lysine-sensitive isoenzyme of aspartokinase (ATP:l-aspartate 4-phosphotransferase, EC 2.7.2.4). Transduction mapping of theaskoperon, inactivated by recombinational insertion of acatmarker, indicates a chromosomal location (about 253°) betweenleuAandaroG. askis thus remote fromaecB, eliminatingaecBas a possible locus for the structural gene of aspartokinase II, but close toaecAanduvrB. The nucleotide sequence of a 2 kb DNA fragment just upstream of theaskoperon was determined and found to contain two open reading frames. The deduced amino acid sequence of the distal reading frame exhibits extensive homology withEscherichia colithioredoxin and that of the proximal one, which overlaps with theaskpromoter, is homologous to the deduced product of theE. coli uvrCgene. Insertional mutagenesis of the proximal open reading frame led to a mitomycin-sensitive phenotype, consistent with a role in DNA repair. In conjunction with the data of M. Petricek, L. Rutberg & L. Hederstedt [FEMS Microbiology Letters61, 85–88] our results define the nucleotide sequence of an 8·8 kb segment of theB. subtilischromosome near 253 ° and the following order of genes:trx-uvrB-ask-orfX-sdhC-sdhA-sdhB-orfY-gerE.