Tolerogenic DNA Vaccine for Prevention of Autoimmune Ovarian Disease
Tolerogenic DNA Vaccine for Prevention of Autoimmune Ovarian Disease
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DOI：
10.3109/08820139.2011.622828
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Wang, Bin
中科院分区：
文献类型：
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作者：
Kang, Youmin;Zheng, Guoxing;Wang, Bin
DNA疫苗已广泛用于诱导针对分子靶点的免疫反应。在这项研究中，我们探讨了使用DNA疫苗联合免疫抑制剂FK 506（他克莫司）的可能性，以抗原特异性抑制不必要的免疫反应，预防自身免疫性卵巢疾病。为此，我们用编码小鼠透明质酸3（ZP 3）的DNA疫苗连同FK 506免疫C57 BL/6小鼠。免疫诱导ZP 3特异性CD 4（+）CD 25（+）Foxp（3+）调节性T细胞（Treg），其抑制动物中ZP 3特异性迟发型超敏反应的诱导。值得注意的是，免疫还保护动物免受实验诱导的自身免疫性卵巢疾病。这些结果表明，在FK 506存在下的DNA疫苗接种可用于诱导Treg细胞并预防AOD。
DNA vaccines have been widely used to induce immune responses against molecular targets. In this study, we explored the possibility of using DNA vaccine combined with the immunosuppressant FK506 (tacrolimus) to antigen-specifically suppress unwanted immune responses and prevent autoimmune ovarian disease. To that end, we immunized C57BL/6 mice with a DNA vaccine encoding mouse zona pellucida 3 (ZP3) together with FK506. The immunization induced ZP3-specific CD4(+)CD25(+)Foxp(3+) regulatory T cells (Treg), which suppressed the induction of ZP3-specific delayed-type hypersensitivity in the animals. Significantly, the immunization also protected the animals from experimentally induced autoimmune ovarian disease. These results suggest that DNA vaccination in the presence of FK506 may be used to induce Treg cells and prevent AOD.