Exercise and injury increase chondroitin sulfate chain length and decrease hyaluronan chain length in synovial fluid
Exercise and injury increase chondroitin sulfate chain length and decrease hyaluronan chain length in synovial fluid
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DOI：
10.1016/j.joca.2007.04.005
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发表时间：
2007-11-01
影响因子：
7
通讯作者：
Merritt, K. A.
中科院分区：
文献类型：
--
作者：
Brown, M. P.;Trumble, T. N.;Merritt, K. A.
目的：(1)研究运动和骨软骨（OC）损伤对滑液（SF）硫酸软骨素（CS）和透明质酸（HA）浓度和链长度的影响；(2)比较OC碎裂关节的SF和软骨CS数据；(3)比较同一马血清中SF CS和HA的数据。方法：分别从(1)休息8周后的正常马、(2)跑步机训练9个月后的同一匹马和(3)比赛中OC损伤的马中提取血清和SF。同时采集3组马的关节软骨。用凝胶色谱法和荧光团辅助碳水化合物电泳法测定CS和HA的浓度和链长。结果：与休息的马（11.6 kDa）相比，OC损伤组的SF - CS峰链长度显著增加（18.7 kDa），运动产生中间链长度（15.6 kDa）。OC损伤组的软骨和血清在这些马的SF中有异常长的CS链。与休息组相比，OC损伤组的总SF HA明显降低。与休息组相比，OC损伤组和运动组的SF HA链长度均显著减少。结论：运动和OC损伤使SF - CS链长增加。运动导致适度增加，而OC损伤导致显著增加。与CS相比，SF HA链长度因OC损伤而减少，运动减少的程度较小。SF - CS和HA的链长分析可为评价关节健康提供有用的工具。(c) 2007年国际骨关节炎研究学会。Elsevier Ltd.出版。版权所有。
Objectives: (1) To investigate the effects of exercise and osteochondral (OC) injury on synovial fluid (SF) chondroitin sulfate (CS) and hyaluronan (HA) concentration and chain length, (2) to compare SF and cartilage CS data from joints with OC fragmentation, and (3) to compare SF CS and HA profiles with those seen in serum from the same horses. Methods: Serum and SF were obtained from (1) normal horses after 8 weeks rest, (2) the same horses after 9 months treadmill training, and (3) horses with OC injury from racing. Articular cartilage was also collected from group 3 horses. Concentrations and chain lengths of CS and HA were determined by gel chromatography and fluorophore-assisted carbohydrate electrophoresis. Results: SF CS peak chain length in the OC injury group increased significantly (18.7 kDa) when compared to rested horses (11.6 kDa), with exercise producing an intermediate chain length (15.6 kDa). Cartilage and serum from the OC injury group had the abnormally long CS chains seen in SF from these horses. Total SF HA was significantly lower in the OC injury group compared to the rested group. Both the OC injury group and the exercised group had significant decreases in SF HA chain length compared to the rested group. Conclusions: Chain length of SF CS was increased by exercise and OC injury. Exercise resulted in a modest increase, whereas OC injury caused a marked increase. In contrast to CS, SF HA chain length was decreased by OC injury, and to a lesser extent by exercise. Chain length analysis of SF CS and HA may provide a useful tool for evaluation of joint health. (c) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.