Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing
Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing
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DOI：
10.3390/app9163380
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发表时间：
2019-08-01
影响因子：
2.7
通讯作者：
Rea, Ilaria
中科院分区：
文献类型：
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作者：
Chianese, Giovanna;Terracciano, Monica;Rea, Ilaria
在这项工作中，纳米结构的氟掺杂氧化锌（nZnOF）的表面功能化与蛋白质A（PrA），并作为一个模型生物分子。化学过程的特征在于几种分析技术，如傅立叶变换红外光谱，水接触角分析，和荧光显微镜。还通过两种无标记光学技术研究了通过结合增加浓度的PrA对nZnOF的表面改性，即，光谱反射法和稳态光致发光法。结果进行了比较，使用未掺杂的nZnO衬底，以突出由于氟掺杂的nZnOF的更好的性能。本研究结果为ZnO基纳米结构无标记光学传感平台的设计和实现奠定了基础。
In this work, the surface of nanostructured fluorine-doped ZnO (nZnOF) is functionalized with protein A (PrA), and used as a model biomolecule. The chemical procedure is characterized by several analytical techniques such as Fourier Transform Infrared Spectroscopy, water contact angle analysis, and fluorescence microscopy. The surface modification of nZnOF by binding increasing concentrations of PrA is also investigated by two label-free optical techniques, i.e., the spectroscopic reflectometry and the steady-state photoluminescence. The results are compared with those obtained using undoped nZnO substrates in order to highlight the better performances of nZnOF due to the fluorine doping. The results of this study pave the way for the design and realization of a ZnO-based nanostructured platform for label-free optical sensing.