Population structure and systematics of Opsariichthys bidens (Osteichthyes: Cyprinidae) in south-east China using a new nuclear marker:: the introns (EPIC-PCR)
Population structure and systematics of Opsariichthys bidens (Osteichthyes: Cyprinidae) in south-east China using a new nuclear marker:: the introns (EPIC-PCR)
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DOI：
10.1111/j.1095-8312.2006.00563.x
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发表时间：
2006-01-01
影响因子：
1.9
通讯作者：
Zhang, CG
中科院分区：
文献类型：
--
作者：
Berrebi, P;Retif, X;Zhang, CG
中国的养鱼业是世界上最古老的水产养殖业。目前野生鱼类动物群及其多样性面临的压力正威胁着水产养殖，因为潜在的祖先经常在野外被捕获。应对这一威胁的可能对策之一是为水产养殖提供新的天然鱼类类群。本研究的目的是分析 Opsariichthys bidens 种群的遗传结构，并使用核标记描述其细分（如果有），以作为该物种潜在水产养殖中种群选择和管理的指南。 2002年和2003年，进行了两次采集之旅，一次在湖南省长江流域中部，另一次在中国广西省西江流域。在 24 个系统上分析了内含子扩增的长度多态性 (EPIC-PCR)，其中只有 5 个给出了易于解释的多态性模式，对应于 11 个假定基因座。根据多维统计，遗传分析表明O. bidens中存在四个明显独立的地理类群，其特征是具有明确的诊断内含子位点。这些类群的分布证实了两个流域种群之间的相似性：珠江流域的长江中部和西江样本。该研究的另一个成果是选择在中国养鱼场首先测试的每个群体的种群。 (c) 2006 年伦敦林奈学会。
Chinese fish farming is the oldest aquaculture in the word. The present pressure on the wild ichthyofauna and its diversity is threatening aquaculture because potential genitors are often caught in the wild. One of the possible responses to this threat is to provide new natural fish taxa for aquaculture. The objective of this study was to analyse the genetic structure of populations of Opsariichthys bidens and to describe its subdivisions, if any, using nuclear markers, to serve as a guideline for stock selection and management in the potential aquaculture of this species. In 2002 and 2003, two collecting trips were made, one in the middle Chang Jiang basin in Hunan Province, and another in the Xi Jiang basin in Guangxi Province, China. Length polymorphisms of the intron amplification (EPIC-PCR) were analysed on 24 systems, only five of which gave easily interpretable and polymorphic patterns, corresponding to 11 presumptive loci. According to the multidimensional statistics, the genetic analysis demonstrated the existence of four clearly independent geographical taxa included in O. bidens, characterized by unambiguous diagnostic intron loci. The distribution of these taxa confirms a similarity between both catchment populations: the middle Chang Jiang and the Xi Jiang samples of the Zhu Jiang basin. An additional output of the study was the choice of the population of each group to be first tested in Chinese fish farms. (c) 2006 The Linnean Society of London.