Aggravated reperfusion injury in STZ‐diabetic nerve
Aggravated reperfusion injury in STZ‐diabetic nerve
复制标题
STZ-糖尿病神经再灌注损伤加重
DOI：
10.1046/j.1529-8027.2002.02004.x
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发表时间：
2002
影响因子：
3.8
通讯作者：
P. McMorran
中科院分区：
文献类型：
--
作者：
H. Nukada;C. Lynch;P. McMorran
摘要 链脲佐菌素-糖尿病神经表现出对叠加急性缺血损伤的形态易感性增加，再灌注损伤加重了缺血神经的病理改变。为了确定STZ糖尿病大鼠神经是否对再灌注敏感，我们评估了20周STZ糖尿病大鼠坐骨神经缺血2.5小时、再灌注3小时和24小时后的病理后果。再灌流3小时后，糖尿病神经出现神经内膜水肿，而非糖尿病对照组出现轻度或无水肿。神经内膜水肿的形态计量学分析(通过横束总面积和神经内膜无结构间隙的点数计分来量化)证实，糖尿病神经中再灌注引起的大腿和膝关节水平的水肿明显多于对照组。再灌流后，糖尿病大鼠大腿水平的神经内膜肥大细胞数量明显增加。再灌流24小时后，糖尿病神经有髓神经纤维形态明显异常，对照组无明显变化。综上所述，我们证明了STZ-糖尿病加重了再灌流的形态改变。因此，糖尿病使微血管系统更容易受到缺血/再灌流的有害影响。
Abstract The streptozocin (STZ)‐diabetic nerve manifests increased morphological susceptibility to a superimposed acute ischemic injury, and reperfusion injury exaggerates ischemic nerve pathology. To determine whether STZ‐diabetic nerves are susceptible to reperfusion, we evaluated the pathological consequences after 2.5 hours of ischemia followed by 3 and 24 hours of reperfusion in a 20‐week STZ‐diabetic rat sciatic nerve. After 3 hours of reperfusion, endoneurial edema developed in diabetic nerves, whereas non‐diabetic controls showed mild or no edema. Morphometric analysis of endoneurial edema, quantified by the total transverse fascicular area and the point‐count score of endoneurial structureless space, confirmed significantly more reperfusion‐induced edema at thigh and knee levels in diabetic nerves than in controls. Reperfusion caused a significant increase in the number of endoneurial mast cells at the thigh level in diabetic nerves. After 24 hours of reperfusion, there were striking morphological anomalies of myelinated nerve fibers in diabetic nerves, without any observable changes in control nerves. In conclusion, we have demonstrated that STZ‐diabetes exacerbates the morphological change to reperfusion. Diabetes therefore renders the microvasculature more vulnerable to the deleterious effects of ischemia/reperfusion.
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影响因子：
37.8
作者：
Panes, J;Kurose, I;Granger, N
通讯作者：
Granger, N
DOI：
--
发表时间：
1984
期刊：
The American journal of pathology
影响因子：
--
作者：
Nukada,H;Dyck,PJ
通讯作者：
Dyck,PJ
影响因子：
14.5
作者：
TUCK, RR;SCHMELZER, JD;LOW, PA
通讯作者：
LOW, PA
DOI：
10.1097/00005072-199756060-00001
发表时间：
1997-06
影响因子：
3.2
作者：
K. Dines;H. Powell
通讯作者：
K. Dines;H. Powell
糖尿病缺血再灌注炎症反应增强的机制。
DOI：
10.1152/ajpheart.1998.275.5.h1773
发表时间：
1998
期刊：
The American journal of physiology
影响因子：
--
作者：
Salas,A;Panés,J;Elizalde,JI;Casadevall,M;Anderson,DC;Granger,DN;Piqué,JM
通讯作者：
Piqué,JM