Single nucleotide polymorphism analysis of exons 3 and 4 of IGF-1 gene in pigs
Single nucleotide polymorphism analysis of exons 3 and 4 of IGF-1 gene in pigs
复制标题
猪IGF-1基因外显子3和4的单核苷酸多态性分析
DOI：
10.4238/vol10-3gmr1328
复制
发表时间：
2011-01-01
影响因子：
0.4
通讯作者：
Jiang, H. Z.
中科院分区：
文献类型：
--
作者：
Hao, L. L.;Yu, H.;Jiang, H. Z.
IGF-1基因被认为是调控猪生长性状的候选基因。约克郡种（50）采用PCR-SSCP方法。外显子3有3个基因型，外显子4有6个基因型，其中外显子3有1个单核苷酸多态性G201A，外显子4有2个单核苷酸多态性A440G和T455C。基因型频率统计分析显示，A等位基因在大猪G201A位点占优势（PIC = 0.20 ~ 0.34）， at等位基因在大猪A440G和T455C位点占优势（PIC = 0.30 ~ 0.60）。各组间基因型分布差异极显著（P < 0.01），均木猪杂合度最高，为0.223，约克郡猪最低，为0.098。各猪品种的IGF-1基因多态性和杂合性分析结果表明，IGF-1具有明显的多态性，且在不同猪品种间的多态性分布和表达水平存在显著差异。这些信息为研究小型猪的遗传背景提供了理论依据，同时也为培育优良猪品种提供了手段。
The IGF-1 gene has been implicated as a candidate gene for the regulation of pig growth traits. We analyzed exons 3 and 4 of IGF-1 gene polymorphisms of the Banna mini-pig (28), the Tibetan mini-pig (30), the Junmu pig (55), and L. Yorkshire species (50) using PCR-SSCP. Three genotypes in exon 3 and 6 genotypes in exon 4 were observed, among which, one single nucleotide polymorphism, G201A, on exon 3 and two single nucleotide polymorphisms, A440G and T455C, on exon 4 were found. Statistical analysis of genotype frequencies revealed that the A allele was dominant in the large pig at the G201A locus (PIC = 0.20-0.34), and the AT alleles were dominant in the large pig at the A440G and T455C loci (PIC = 0.30-0.60). The genotype distribution between the various groups was significantly different (P < 0.01), with the highest heterozygosity seen in Junmu pigs at 0.223 and the lowest seen in L. Yorkshire at 0.098. The genetic distance of the Junmu pig from the L. Yorkshire is the smallest, the distance from the Tibetan miniature pigs is larger, and the distance from the Banna minipig is the largest. The IGF-1 gene polymorphism and heterozygosity results from various pig breeds indicate that IGF-1 is substantially polymorphic with significant difference of the polymorphic distribution and expression levels among various pig breeds. This information provides a theoretical basis for the genetic background of miniature pigs but also provides means to breed improved pig varieties.