Hrb27C, Sqd and Otu cooperatively regulate gurken RNA localization and mediate nurse cell chromosome dispersion in Drosophila oogenesis
Hrb27C, Sqd and Otu cooperatively regulate gurken RNA localization and mediate nurse cell chromosome dispersion in Drosophila oogenesis
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DOI：
10.1242/dev.01078
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发表时间：
2004-05-01
期刊：
影响因子：
4.6
通讯作者：
Schüpbach, T
中科院分区：
文献类型：
--
作者：
Goodrich, JS;Clouse, KN;Schüpbach, T
异质核核糖核蛋白（hnRNP）是RNA结合蛋白，在控制基因表达中起关键作用。在果蝇卵子发生过程中，hnRNP Squid（Sqd）参与了gurken（grk）mRNA的定位和翻译调控。我们发现，Sqd与Hrb27C相互作用，Hrb27C是一种以前参与剪接的hnRNP。与sqd一样，hrb27C突变体产下具有背腹缺陷的卵，并且hrb27C可以直接结合grk RNA。我们的数据表明Hrb27 C在促进grk定位中的新作用。我们还观察到Hrb27 C和卵巢肿瘤（Otu），一种参与RNA定位的细胞质蛋白之间的直接物理相互作用。我们发现，一些otu等位基因产生背侧化的卵，它出现Otu合作与Hrb27C和Sqd在卵母细胞介导适当的grk定位。所有三种突变体都有另一种表型，即持久多线营养细胞染色体。我们的分析支持在果蝇卵子发生过程中的Sqd，Hrb27 C和Otu的双重合作作用。
Heterogeneous nuclear ribonucleoproteins, hnRNPs, are RNA-binding proteins that play crucial roles in controlling gene expression. In Drosophila oogenesis, the hnRNP Squid (Sqd) functions in the localization and translational regulation of gurken (grk) mRNA. We show that Sqd interacts with Hrb27C, an hnRNP previously implicated in splicing. Like sqd, hrb27C mutants lay eggs with dorsoventral defects and Hrb27C can directly bind to grk RNA. Our data demonstrate a novel role for Hrb27C in promoting grk localization. We also observe a direct physical interaction between Hrb27C and Ovarian tumor (Otu), a cytoplasmic protein implicated in RNA localization. We find that some otu alleles produce dorsalized eggs and it appears that Otu cooperates with Hrb27C and Sqd in the oocyte to mediate proper grk localization. All three mutants share another phenotype, persistent polytene nurse cell chromosomes. Our analyses support dual cooperative roles for Sqd, Hrb27C and Otu during Drosophila oogenesis.