Factors determining the fluorescence yield of fucoxanthin-chlorophyll complexes (FCP) involved in non-photochemical quenching in diatoms
Factors determining the fluorescence yield of fucoxanthin-chlorophyll complexes (FCP) involved in non-photochemical quenching in diatoms
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DOI：
10.1016/j.bbabio.2012.03.008
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发表时间：
2012-07-01
影响因子：
4.3
通讯作者：
Buechel, Claudia
中科院分区：
文献类型：
--
作者：
Gundermann, Kathi;Buechel, Claudia
从中心硅藻Meneghiniana中分离出岩藻黄质-叶绿素复合体(FCP)，并以不同的脂类与Chla比例将三聚体FCPA复合体重组为脂质体。根据室温荧光发射光谱计算了配合物在不同环境中的荧光产额，并与FCPA的聚集态进行了比较。被大量脂类包围的FCPA类似于洗涤剂增溶的复合体，随着脂类水平的下降，即在蛋白质接触日益有利的情况下，FCPA的荧光产量逐渐减少。此外，在油脂含量较低的情况下，产率表现出强烈的pH依赖性。双黄素转化为双黄素所带来的荧光产率的进一步降低与pH无关，且仅与双黄素的合成量有关。讨论了这些数据对中心硅藻的非光化学猝灭的影响。(C)2012爱思唯尔B.V.保留所有权利。
Fucoxanthin-chlorophyll complexes (FCP) from the centric diatom Cyclotella meneghiniana were isolated and the trimeric FCPa complex was reconstituted into liposomes at different lipid to Chl a ratios. The fluorescence yield of the complexes in different environments was calculated from room temperature fluorescence emission spectra and compared to the aggregated state of FCPa. FCPa surrounded by high amounts of lipids resembled detergent solubilised complexes and with decreasing lipid levels, i.e. in a situation where protein contacts were increasingly favoured, the fluorescence yield of FCPa gradually decreased. In addition, the yield displayed a strong pH-dependency in case of lower lipid contents. The further reduction in fluorescence yield brought about by the conversion of diadinoxanthin to diatoxanthin was pH independent and only depended on the amount of diatoxanthin synthesised. The implications of these data for nonphotochemical quenching in centric diatoms are discussed. (C) 2012 Elsevier B.V. All rights reserved.