Identification of an antigenic domain in the N-terminal region of avian hepatitis E virus (HEV) capsid protein that is not common to swine and human HEVs
Identification of an antigenic domain in the N-terminal region of avian hepatitis E virus (HEV) capsid protein that is not common to swine and human HEVs
复制标题
禽戊型肝炎病毒 (HEV) 衣壳蛋白 N 末端区域中猪和人 HEV 不常见的抗原域的鉴定
DOI：
10.1099/vir.0.069021-0
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发表时间：
2014-12-01
影响因子：
3.8
通讯作者：
Zhou, En-Min
中科院分区：
文献类型：
--
作者：
Wang, Lizhen;Sun, Yani;Zhou, En-Min
对鸡戊型肝炎病毒(HEV)衣壳蛋白C端268个氨基酸残基的抗原区进行了研究。该地区与猪和人的HEV有共同的表位。然而，N端338个氨基酸残基的表位尚未见报道。本研究以截短的重组衣壳蛋白为包被抗原，以抗禽HEV鸡血清为一抗，用间接ELISA法鉴定了位于23~85位氨基酸之间的抗原区。此外，该结构域不与抗猪和人HEV血清反应。这些结果表明，禽HEV衣壳蛋白N端338个氨基酸残基与猪和人HEV不具有共同的表位。这一发现对于我们理解禽类HEV衣壳蛋白的抗原性具有重要意义。此外，它对血清学诊断中病毒抗原的选择也有重要意义。
The antigenic domains located in the C-terminal 268 amino acid residues of avian hepatitis E virus (HEV) capsid protein have been characterized. This region shares common epitopes with swine and human HEVs. However, epitopes in the N-terminal 338 amino acid residues have never been reported. In this study, an antigenic domain located between amino acids 23 and 85 was identified by indirect ELISA using the truncated recombinant capsid proteins as coating antigens and anti-avian HEV chicken sera as primary antibodies. In addition, this domain did not react with anti-swine and human HEV sera. These results indicated that the N-terminal 338 amino acid residues of avian HEV capsid protein do not share common epitopes with swine and human HEVs. This finding is important for our understanding of the antigenicity of the avian HEV capsid protein. Furthermore, it has important implications in the selection of viral antigens for serological diagnosis.