MicroRNA expression profile by next-generation sequencing in a novel rat model of contrast-induced acute kidney injury
MicroRNA expression profile by next-generation sequencing in a novel rat model of contrast-induced acute kidney injury
复制标题
通过下一代测序在新型造影剂诱导的急性肾损伤大鼠模型中显示 MicroRNA 表达谱
DOI：
10.21037/atm.2019.04.44
复制
发表时间：
2019-04-01
影响因子：
--
通讯作者：
Chen, Jiyan
中科院分区：
文献类型：
--
作者：
Liu, Yong;Liu, Bowen;Chen, Jiyan
背景：已知microRNA（miRNAs）调节大多数生物学过程，包括造影剂诱导的急性肾损伤（CI-AKI）。很少有研究探讨microRNA（miRNA）的表达在一个新的大鼠模型CI-AKI使用非离子型低渗碘造影剂优维显，并进行基因本体论（GO）categories analysis.Methods：在这项研究中，肾组织收集从Sprague-Dawley大鼠在24小时后造影剂暴露（CI-AKI）和生理盐水管理（对照）。使用miRNAs微阵列分析通过下一代测序检测肾组织中的miRNAs。实时荧光定量PCR验证芯片检测结果。通过GO类别和京都基因和基因组百科全书（KEGG）途径分析所有显着差异表达的miRNA。结果：在检测到的173种miRNA中，22种下调（miR-328 a-5 p、miR-31 a-5 p、tniR-377- 3 p等），19种上调（miR-3558- 5 p、miR-34 c-3 p、miR-384- 5 p等）。根据log 2（倍数变化，对照组）>1，CI-AKI大鼠肾脏中，P
Background: MicroRNAs (miRNAs) are known to regulate most biological processes including contrast-induced acute kidney injury (CI-AKI). Few studies have investigated microRNA (miRNAs) expressions in a novel rat model of CI-AKI using nonionic low-osmolar iodic contrast medium Ultravist, and performed gene ontology (GO) categories analysis.Methods: In this study, kidney tissues were collected from Sprague-Dawley rats at 24 h after contrast-exposure (CI-AKI) and saline-administration (control). MiRNAs microarray assays were used to detect miRNAs in the kidney tissue by next-generation sequencing. Real-time PCR was performed to verify the microarray assays results. All significant differently expressed miRNAs were analyzed by GO categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways.Results: Of the 173 detected miRNAs, 22 were down-regulated (miR-328a-5p, miR-31a-5p, tniR-377-3p, et at) and 19 were up-regulated (miR-3558-5p, miR-34c-3p, miR-384-5p, et a/.) in the kidneys of CI-AKI rats according to log2 (fold change, control) >1, P