OLIGODENDROCYTES IN THE EARLY COURSE OF MULTIPLE-SCLEROSIS
OLIGODENDROCYTES IN THE EARLY COURSE OF MULTIPLE-SCLEROSIS
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DOI：
10.1002/ana.410350111
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发表时间：
1994-01-01
影响因子：
11.2
通讯作者：
LASSMANN, H
中科院分区：
文献类型：
--
作者：
BRUCK, W;SCHMIED, M;LASSMANN, H
在多发性硬化症的早期阶段，通过诊断性针刺活检获得的标本，研究了多发性硬化脱髓鞘病变的神经病理学。病变的特点是以淋巴细胞和巨噬细胞为主的慢性炎症反应，斑块状脱髓鞘和星形胶质硬化。对病变内的少突胶质细胞进行免疫细胞化学研究，使用针对不同髓鞘和少突胶质细胞成分的抗体。用原位杂交法检测蛋白质脂蛋白信使RNA的表达。我们的研究表明，髓鞘少突胶质细胞糖蛋白是鉴定脱髓鞘斑块中少突胶质细胞的敏感和可靠的标记物。结果表明，在疾病的早期，在一些患者中，少突胶质细胞可能大部分保存下来，而在另一些患者中，少突胶质细胞明显丢失。少突胶质细胞的丢失仅与病变内脱髓鞘活动的阶段略有关系。这些发现表明，不同的多发性硬化症患者脱髓鞘的发病机制可能有所不同。
The neuropathology of demyelinating lesions in multiple sclerosis was studied in specimens obtained by diagnostic needle biopsy during early stages of the disease. The lesions were characterized by a chronic inflammatory reaction dominated by lymphocytes and macrophages, plaque-like demyelination, and astroglial sclerosis. Oligodendrocytes within the lesions were studied by immunocytochemistry using antibodies against various myelin and oligodendroglia components. The expression of messenger RNA for proteolipid protein was determined by in situ hybridization. Our studies revealed that myelin-oligodendrocyte glycoprotein is a sensitive and reliable marker for identification of oligodendrocytes in demyelinated plaques. The results suggest that in the early course of the disease in some patients, oligodendrocytes may largely be preserved, whereas in others oligodendroglial loss is pronounced. Loss of oligodendrocytes was only marginally related to the stage of demyelinating activity within the lesions. These findings indicate that the pathogenesis of demyelination may vary within different multiple sclerosis patients.