The mechanism of entry of viruses into plant protoplasts.
The mechanism of entry of viruses into plant protoplasts.
复制标题
病毒进入植物原生质体的机制。
DOI：
10.1002/9780470720639.ch5
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
J. King
中科院分区：
文献类型：
--
作者：
J. Watts;J. Dawson;J. King
植物原生质体可以有效地感染病毒。附着在质膜上是感染的关键步骤；带正电荷的病毒很容易粘附和感染，但带负电荷的病毒在感染发生之前需要多阳离子（例如聚l -鸟氨酸）的存在。聚合的作用似乎是双重的：修饰病毒的电荷和破坏原生质体的表面。病毒可能在质膜受损区域的修复过程中进入原生质体。在接种过程中，胞饮作用似乎不起重要作用。
Plant protoplasts may be efficiently infected with viruses. Attachment to the plasmalemma is a critical step in infection; positively charged viruses readily adhere and infect but negatively charged viruses require the presence of a polycation (e.g. poly-L-ornithine) before infection can occur. The role of the polycation appears to be twofold: to modify the charge of the virus and to damage the surface of the protoplast. Virus probably enters the protoplast during repair of damaged regions of the plasmalemma. Pinocytosis does not appear to play a significant part in inoculation.