A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci.
A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci.
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农杆菌毒力所必需的基因与正调控基因座家族同源。
DOI：
10.1073/pnas.83.21.8278
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发表时间：
1986
影响因子：
11.1
通讯作者：
Nester,EW
中科院分区：
文献类型：
--
作者：
Winans,SC;Ebert,PR;Stachel,SE;Gordon,MP;Nester,EW
根癌农杆菌的vir区域跨越冠瘿肿瘤发生所需的至少六个转录位点。通过使用位于Ti质粒上的六个vir基因座中的每一个中含有突变的细菌来测量响应于与烟草悬浮细胞共培养的这些vir基因座中的两个的转录诱导。这些vir基因的诱导仅发生在具有virA和virG功能拷贝的细菌中。包含virG的1.25-腺苷酸酶克隆的核酸序列含有一个能够编码约30，000道尔顿蛋白质的开放阅读框。预测的virG产物的氨基酸序列与8种细菌蛋白的氨基酸序列同源，包括大肠杆菌ompR基因的氨基酸序列。这些蛋白质中的大多数（尽管不是全部），如VirG，是正调控元件。
The vir region of Agrobacterium tumefaciens spans at least six transcriptional loci required for crown gall tumorigenesis. The transcriptional induction of two of these vir loci in response to cocultivation with tobacco suspension cells was measured by using bacteria containing mutations in each of the six vir loci located on the Ti plasmid. Induction of these vir genes occurred only in bacteria that had functional copies of virA and virG. The nucleic acid sequence of a 1.25-kilobase clone encompassing virG contains one open reading frame capable of coding for a protein of about 30,000 daltons. The amino acid sequence of the predicted virG product is homologous to that of eight bacterial proteins, including that of the ompR gene of Escherichia coli. Most, although not all, of these proteins, like VirG, are positive regulatory elements.