Properties of the electrogenic activity of bacteriorhodopsin.
Properties of the electrogenic activity of bacteriorhodopsin.
复制标题
细菌视紫红质的电活性特性。
DOI：
10.1007/s00249-012-0870-0
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Muneyuki E.
中科院分区：
文献类型：
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作者：
Miyazaki S;Matsumoto M;Brier SB;Higaki T;Yamada T;Okamoto T;Ueno H;Toyabe S;Muneyuki E.
在这项研究中，我们分析了吸附在聚合物薄膜“Lumirror”上的细菌视紫红质产生的光电电流（Muneyuki 等人，FEBS Lett 427:109–114, 1998）。由于聚合物薄膜的机械和化学稳定性，我们可以使用同一膜在较宽的光强度和 pH 值范围内检查光电电流。我们通过与简单的等效电路进行比较来分析光电电流。对不同光强度下获得的实验结果的分析表明，细菌视紫红质的电动势与光强度无关。光电流的 pH 依赖性表明细菌视紫红质在 pH 值约为 6 时可产生最大电动势。
In this study, we analyzed the photoelectric current generated by bacteriorhodopsin adsorbed on a polymer film, “Lumirror” (Muneyuki et al. in FEBS Lett 427:109–114, 1998). We could examine the photoelectric current over a wide range of light intensity and pH values using the same membrane owing to the mechanical and chemical stability of the thin polymer film. We analyzed the photoelectric current by comparison with a simple equivalent electric circuit. Analysis of experimental results obtained at different light intensities suggested that the electromotive force of the bacteriorhodopsin was independent of light intensity. The pH dependence of the photoelectric current suggested that the bacteriorhodopsin could generate a maximum electromotive force at approximately pH 6.